补肾宁神膏的指纹图谱建立、含量测定及体外抗氧化作用的谱效关系研究

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中图分类号 R84.1;R917 文献标志码A 文章编号 1001-0408（05）14-1749-06

DOI10.6039/j.issn.1001-0408.05.14.10

ABSTRACTOBJECTIVEToestablish thefingerprint of Bushenningshenointment，determinethecontentsof its major constituents，andinvestigateitsinvitroantioxidantactivity.METHODsHighperformanceliquidchromatography（HPLC） fingerprintsof1batchesofBushen nngshen ointment were established.Similarityevaluationand identificationofcommonpeaks weresubsequently performed.Thecontentsof1Ocomponentssuchassalidrosidewere determinedusing thesame HPLCmethod. Using the scavenging rates against 2， 2′ -azino bis （3-ethylbenzothiazoline-6-sulfonic acid）（ABTS）and1，1-diphenyl-2- picrylhydrazyl（DPH）adicals，aswellasfericionreducingantioxidantpower（FRAP）asindcators，theanti-oxidantactivityof theointment was evaluated；greyrelationalanalysisandpartialleastsquaresregresion wereconductedusingSMCA14.1software toestablishthespectrum-efectrelationship.RESULTSThefingerprintchromatogramof10batchesof Bushenningshenointment contained 24 common peaks， with similarity valuesallexceeding O.96.Eleven peaks were identified as adenosine（peak1），salidroside（peak4），morroniside（peak6），catechin（peak7），paeoniflorin（peak 1O），spinosin（peak11），ferulic acid （peak 12），isoquercitrin （peak 13），verbascoside（peak 14），paeonol （peak 23），and emodin（peak 24）.Content determination results showed that theaverage contentsof salidroside，morroniside，catechin，paeoniflorin，spinosin，ferulicacid，isoquercitrin，verbascoside，paeonol，andemodinwere0.725，1.962，0.214，3.395，0.124，0107，0.286， 0.019，0.034 and 0.067mg/g ，respectively.The antioxidant potency composite index（APC）for the 10 batches ranged from 85.08% to 96.35% .Spectrum-effect relationshipanalysis indicated that all 24common peaks were positively correlated with the antioxidant capacity. Seventeen peaks had variable importance in projection values >1 ，specitically peaks 2，5，6，7，9，10，13- 21，23，and24.CONCLUSIONSThis studysucesfullyestablishedthe HPLC fingerprintandcontentdetermination method for Bushenningshenointment.Thecompoundsrepresented bythe17commonpeakssuch as moronisidemaybetheactivecomponents contributing to its antioxidant effects.

KEYWORDsBushenningshenointment；fingerprint；spectrum-efectrelationship；antioxidantactivity；contentdetermination

补肾宁神膏根据深圳市中西医结合医院治未病科徐文华主任医师的临床经验方制备而成，该方由熟地黄、山茱萸、酒川芎、盐女贞子、炒酸枣仁、牡丹皮、首乌藤、茯神、龙齿等16味药材组成，具有滋阴补肾、养心安神的功效，临床主要用于治疗肾血不足、虚火内扰所致的失眠、多梦等症，疗效显著。（剩余10295字）