滇紫草与昆明滇紫草HPLC指纹图谱的建立及对比研究

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【中图分类号】R284.1 【文献标志码】A 【文章编号】1007－8517（2025）15-0041-06

DOI： 10.3969/j .issn.1007-8517. 2025.15. zgmzmjyyzz202515009

Establishment and Comparative study of HPLC Fingerprint of Onosma paniculatum and Onosma kunmingense

YANGYimeiGUO Weiyi*LIU XiaojunPANG Shilong ZHANGYuyao Kunming Food and Drug Inspection Research Institute，Kunming 65OoOO，China

Abstract：ObjectiveEstablishtheHPLCfingerprintsofOnosmapaniculatumandOnosmakunmingense，andcomparethediferences between their fingerprints.Methods High performance liquid chromatography：TechMate PFP STV100A（ 4.6mm×250 mm ×5μm ）；mobile phase A is 0.2% formic acid，mobile phase B is acetonitrile，gradient elution，flow rate is 1.0mL/min ，column temperature 35‰ ，and the analytical wavelength is 516nm . The TCM chromatographic fingerprint similarity evaluation system（2004 edition）wasusedtoestablishthefingerprint mapsofOnosmapaniculatumandOnosma kunmingense，calculatethesimilarity，andidenifythecommonpeak withthereferenceproduct.Results TheHPLCfingerprintofOnosma paniculatumshowed8commonpeaks， amongwhich6wereidentified.TheHPLCfingerprintofOnosmakunmingensealsocontained8commonpeaks，with5of themidentified.ThesimilaritybetweenOnosmapaniculatumandOnosmakunmingensewaslow.ConclusionTherearediferencesintheHPLC fingerprintsbetweenOnosmapaniculatumandOnosmakumingense.Thismethodissimpleandhasgoodreproducibilityhichcanbe used for the quality evaluation of Onosma paniculatum and Onosma kunmingense.

Key words：Onosma paniculatum；Onosma kunmingense；HPLC；Fingerprint；Similarity

紫草是我国传统中药材 [1-2] ，含多种萘醌类化合物，此类化合物已被证实具有抗肿瘤、抗菌、抗病毒、抗炎等多种生物活性[3-4]。（剩余6427字）