鲫miR-21靶基因筛选与组织分布及皮肤免疫时序表达分析

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中图分类号：S917.1 文献标志码：A 文章编号：1004—6755（2025）06-0016—03

Screening of target genes and tissue distribution of Carassius auratus miR-21 and temporal expression analysis in skin immunity

JIN Ge，HUO Jiaxin，LIN Xuan， CHEN Yixin， ZHOU Junmin，HU Xiucai，LV Aijun

（AquaticColge，TianjinAgriculturalUniversityTianjinKeyLaboratoryofquaticecologyandBreedingianjino392ina） Abstract：In order to clarify the molecular mechanism of miR-21 involvement in mediating immune related signaling pathways in Carassus auratus skin infected with Aeromonas hydrophila， miR-21 and its target genes were identified through screening and enriched immune related signaling pathway analysis. Real time fluorescence quantitative PCR （qRT PCR） was used to detect tissue distribution and temporal expression levels.The results indicate that the predicted binding site sequences of miR -21 and BCL2 target genes are highly conserved among diferent species such as humans，mice，and fish. QRT PCR detection showed that miR 21 was mainly highly expressed in the gils， intestines，and muscle tissues of Carassius auratus ，in Carassius auratus skin infected with Aeromonas hydrophila，miR -21 exhibited a short-term fluctuating temporal expression pattern; miR -21 regulates the target gene BCL2 to mediate the downregulatied of TLR/MAPK signaling pathway related genes （such as IRAK1， TAB1 ， IKKa ）in the Carassius auratus skin，while TLR4 and RHO genes show significant upregulation at 12 and 72 hours of infection，and BCL2 is significantly correlated with TAB1 gene expression （ r=0.87 ）.The above research results contribute to the understanding of the molecular regulatory mechanisms of miR -21 and target gene functions in fish，as well as its mediation of skin immunity.

Key words： Carassius auratus ; Aeromonas hydrophila ; miR-21 ；target gene； tissue distribution; temporal expression; skin immunity

鱼类miRNA对许多生物学过程如发育、代谢、炎症和疾病产生广泛影响，其中大多数miR-NA在鱼类中的调控机制尚不清楚[1]。（剩余4668字）