谷胱甘肽响应型姜黄素荧光探针的构建及细胞成像

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[关键词］谷胱甘肽；荧光探针；姜黄素；线粒体；活性氧；细胞成像 [中图分类号]R284;R285.5 [文献标志码]A [文章编号]doi：10.3969/j.issn.1674-070X.2025.06.006

Construction and cell imaging of GSH-responsive curcumin probe

YANG Haoyu， ZHOU Xinyi， CHEN Xin， DAI Hongfang， QIN Yan* School ofPharmacy，Hunan University of Chinese Medicine， Changsha，Hunan 4lO2O8，China

[Abstract]ObjectiveTosynthesize a glutathione （GSH）-responsivecurcumin-based fluorescent probe （CPB）andapply it forhepatocellarcarcinomacellimaging，investigatingitscytotoxcitymitochondria-argetingcapability，andabilitytoinduce reactiveoxygen species （ROS）generation Methods A GSH-responsive fluorescent probe （CPB）was synthesized using curcuminasa fluorescentmoietyanditsstructurewascharacterizedbyliquidchromatographyassspectrometry（C-MS）anduclearmagnetic resonance （NMR）.Ultraviolet （UV）spectroscopywasused toanalyzeitsresponsetoGSHandtimedependentchanges.Thecytotoxicity ofCPBwasaessdbsaynditsgetielodioiaonicellagingdhodalcalatio experiment.Results Theresultsof NMRcarbon spectraandLC-MSconfirmed thesuccesful synthesisof CPB.UVspectra showedthattheresponsesignalofCPBitself wasconcentration-dependent，whilethatofCPBtoGSHwastime-dependent.MTT assay showed that CPB of 20-30μmol/mL significantly inhibited the proliferation of HepG2 cells，and CPB of 40μmol/mL （20 inhibitedtheproliferationofBEL7402/DPcells，whileshowingalmostnotoxicitytoAML-12cels，indicatinglowcytotoxicity.ROS assay showed that CPBinducedROS generationin HepG2andBEL7402/DDPcelsinaconcentration-dependent manner. Comparedwiththecontrol group，thecellimaging experiment demonstrated that thefluorescence intensityof CPBenhancedat concentrationsof10＼～3Oμmol/Landitsfluorescencesignalslargelyoverlappedwiththoseofcommercialmitochondrialprobes. Conclusion Inthispaper，CPB，aGSH-responsivecurcumin-basedfluorescentprobe，wasdesignedandsynthesized.Itexhibits goodGSHresponsesensitivitylowcytotoxicity，andmitochondrialtargeting，whichcanbeappliedasasafeandreliable GSH detection probe for tumor cell imaging.

[Keywords] glutathione; fluorescent probe; curcumin; mitochondria; reactive oxygen; cell imaging

肝癌作为一种全球范围内常见且极具侵袭性的疾病，严重威胁着人类的生命健康[1-2]。（剩余11053字）