去甲乌药碱配伍6-姜酚前后对衰竭心肌细胞影响的研究

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[关键词]去甲乌药碱；6-姜酚；H9c2心肌细胞；能量代谢；线粒体 [中图分类号]R285.5 [文献标志码]A [文章编号]doi：10.3969/j.issn.1674-070X.2025.04.009

[Abstract]ObjectiveTocomparetheefectsofhigenamine beforeandaftercombinationwith6-gingerolon failing cardiomyocytes.Methods A failing H9c2 cell model was established using hydrogen peroxide （ H2（ρ）2） .The cells were divided into nine groups： blank group，model group， 6-gingerol group，higenamine single-use groups （5，10，20 μmol/l ），and higenamine combined with 6-gingerol （20 μmol/L ）groups.The mitochondrial morphology of the cellswas observed，and the mitochondrial membrane potential， Ca2+ concentration，ATP content，and mitochondrial membrane-associated active transport enzyme activity were measured. Results The replication conditions for inducing failing H9c2 cells were determined as 1000 μ mol/L H2O2 for2 hours.Comparedwiththemodelgroup，bothhigenaminemonotherapyanditscombinationwith6-gingerolsignificantly aleviatedmitochondrialulrastructuraldamage.Comparedwiththeuseof higenaminealone，thecombinationwith6-gingerol hadasynergisticprotectiveefectonthemodelcells，asevidencedbyamoreintactmitochondrialmembrane，clearer boundaries，andrestoredcristaestructure.Additionall，mitochondrialmembranepotentialwassignificantlyhigher，cytoplasmic and mitochondrial Ca2+concentrations were markedly lower，andATP content，along withtheactivities of Na+- K+. -ATPase， Ca 2+， -ATPase，and Ca 2+， -Mg2+-ATPase， significantly enhanced （ P<0.05 ， P<0.01 ）. Conclusion The combination of higenamine and 6- gingerolcansignificantlyenhancetheprotectiveefectonfaiing H9c2cardiomyocytes.Thisefectmayberelatedtotheir synergisticactioninalleviatingmitochondrialcalciumoverload，increasingmitochondrialATPaseactivity，promotingATP production，and restoring mitochondrial function.

[Keywords] higenamine;6-gingerol; H9c2 cardiomyocytes；energy metabolism；mitochondria

附子-干姜为经典药对，两者相须配伍具有增效作用，素有“附子无干姜不热之说"。（剩余10577字）