中等强度耐力运动对老年性骨质疏松症小鼠NLRP3/Caspase-1/GSDMD焦亡通路的影响

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[关键词]老年性骨质疏松症；耐力运动；炎症；焦亡;NLRP3;Caspase-1;GSDMD [中图分类号]R68;R274 [文献标志码]A [文章编号]doi：10.3969/j.issn.1674-070X.2025.04.005

[Abstract]ObjectiveToinvestigatetheefectsof moderate-intensityenduranceexerciseonsenileosteoporosis （SOP） throughtheNOD-likereceptor family pyrindomaincontainingprotein3 （NLRP3）/cysteineprotease-1 （Caspase-1）gasdermin D （GSDMD）signaling pathway.Methods Twenty-one18-month-old maleC57mice wererandomlydivided into three groups：the18- month-oldgroup，the2O-month-oldgroup，andtheexercisegroup，with seven mice ineach group.The SOPanimal model was replicatedusing naturallaged malemice，andanadditional seven six-month-old maleC57mice wereselectedas theyounggroup. Afteroneweek ofadaptivefeding，miceintheyoung groupand18-month-oldgroupwereeuthanized forsamplecolection.Mice intheexercisegroupunderwentmoderate-intensityendurancetraining，whilethoseinthe2O-month-oldgroupreceivedno intervention.Samples werecoletedaftereightweeksof intervention.Bone mineral density（BMD）of theleftfemur ineachgroup of micewasmeasuredusing micro-computedtomography（Micro-CT.ELISAwasused todeterminetheserum levelsof bone metabolismmarkers，ncludingC-terminaltelopeptideoftypeIcollgen（CTX-1），tartrateresistantacidphosphatase（AC） terminal propeptdeoftyeIprocollgen（INP），andbospcificalalinephospatase（B-APInditin，levelsofitein 1β（IL-1β）andinterleukin-18（IL-18）intherightfemurwereasessedWesternblotanalysiswasconductedtodeteminethe proteinexpresionlevelsofNRP3，Caspase-1，andtheN-terminalfragmentofGSDMD-Nintherightfemurofeachgroup. Results Compared with theyoung group，the18-month-oldand2O-month-old groups showed increased serum levelsof CTX-1and TRACP，as well as elevated expression levels of NLRP3，Caspase-1，GSDMD-N， IL- 1β ，andIL-18intherightfemur（ P <0.05， P< 0.01）.Meanwhile，serum levels of PINP and B-ALP，along with BMDof the left femur，decreased （ P <0.05， P≪0.01 1）.Compared with the18-month-oldgroup，the2O-month-old group exhibited increases inserum CTX-1andTRACPlevels，aswellasinNLRP3， Caspase-1， GSDMD-N， IL-1β，and IL-18 expression levels in the right femur （ P≪0.05 P≪0.01 ），along with decreases in serum PINP and B-ALP levels and left femur BMD （ P <0.05， P <0.01）.Compared with the 2O-month-old group， the exercise group showed reductions inerumCTX-1andRACPlevels，aswellasinrightfemoralexpresionlevelsofLRP3，Caspase-1，GD-N，IL 1β，and IL-18 （ P <0.05， P

老年性骨质疏松症（senileosteoporosis，SOP）是一种与增龄相关的代谢性骨病，主要表现为骨量低下、骨组织微结构损坏而导致骨脆性增加，易发生骨折2。（剩余13474字）