油菜花分生组织特征基因LFY的克隆及生信分析

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中图分类号：Q811.4；S565.4 文献标识码：A 文章编号：1006-060X（2025）08-0007-04

Cloning and Bioinformatics Analysis of LFYInvolved in Floral Meristem of Brassica napus

ZHONG Kai'， ZHANG Long-yan²，LI Wei³，HUANG Hu-lan4，LIU Wen-xiang

（1. ZiyangDistrictBureauofAgricultureandRuralAffairs，Yiyang4l3oo，PRC;2.AgriculturalComprehensieSeruiceCenterof ZhijingDongutonomousountyZijiang49ioo，RC;3.AgriculturalcienceandechologyerviceCenterofHroce Changsha41O06，PRC;4.CroResearchInstuteofHunanProvince，Changsha41025，PRC;5.HunanIstituteofAgicultual Economics and Information， Changsha 410125，PRC）

Abstract：This studycloned BnaA02.LFYfrom Brassica napus and predicted the physiochemical properties，conserveddomains， and secondary structure of the deduced protein. The results showed that BnaAO2.LFY wascomposed of 420 amino acid residues and BnaAO2.LFY protein had a relative molecularweight of 46.5kDa ，anisoelectricpoint of6.29，aninstabilityindexof 55.34（poor stability），agrand averageofhydropathicityof -0.62 （goodhydrophilicity），and analiphatic indexof 68.61.The secondary structure analysis showed that alpha-helices，randomcoils，extended strands，and beta-turns accounted for 43.86% 38.31% 5 10.12% ，and 7.71% respectively.Theclusteranalysis basedonamino acid sequences showedthat BnaAO2.LFYsharedthesameclade with homologous sequencesfrossicacaaicintaascecdsisicatingeiosep.

Keywords：LFY;Brassica napus;genecloning;bioinformaticsanalysis

油菜（BrassicanapusL.）是我国自产植物油第一大油源[1-2]，产油量占国产植物油的 50% 左右[3]。（剩余5351字）