辣椒花粉生活力快速测定比较

打开文本图片集

关键词：辣椒；花粉；花形态；生活力；染色方法

中图分类号：S641.3 文献标志码：A 文章编号：2097-2172（2025）11-1036-04

doi：10.3969/j.issn.2097-2172.2025.11.010

Comparison ofRapidDetermination Methodsfor ChiliPepper Pollen Viability

ZHANG Ru （VegetableResearch Institute，Gansu Academy of Agricultural Sciences，Lanzhou Gansu 73oo70，China）

Abstract：Toidentifyarapid，efective，ndhighlyaccuratechemicalstaining methodformeasuring polen viabity inchili pepper，andtodeterminetheoptimalperiodforpolenviabilityasessment，therebyprovidingareferenceforimprovinghybrid polinationefciencyandfruitsetateinchilipeppr，4chilipepperinbredlines，C123，Y32C131，andC125，wereselected. Pollenviability was evaluated using the acetocarmine staining method， l2 -IKstaining method，and Alexander staining method. Meanwhile，pollenviabilitywasalsoasessedfrom3floraldevelopmentalstages：largeflowerbudsbeforeanthesis，flowersat anthesiswithoutpollendispersal，andflowersatathesiswithpolendispesalResultsshowedthattheaveragepollenvbilis detectedbytheacetocarmine method，theI2-IK method，and theAlexander method were 91.71% ， 50.20% ，and 81.33% ，respectively. The acetocarmine and l2 -IK methods identified viable pollen basedonlyonasingle criterion，color or morphology，often resulting in overestimationoruderestimationofviability.Incontrast，theAexandermethodevaluatedbothcolorandmorphologyfectiely reducingselectionbias.Polenviabilityacrossthe3flowertypesrankedfromhighesttolowestas：flowersatanthesiswithoutpolen dispersal，flowsisiolispsaldledfoesislectigfosattppropateeotal stage for pollination can significantly improve the fruit set rate in chili pepper.

KeyWords：Chili pepper;Pollen; Flower morphology; Vitality; Chemical staining method

辣椒（CapsicumannuumL.）起源于南美洲，隶属于茄科辣椒属，是全球广泛栽培的重要蔬菜作物[1-3]。（剩余5963字）