双修饰三螺旋DNA荧光分子开关高灵敏检测玉米 Bt 转基因

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Abstract：The Bacillus thuringiensis （Bt）gene，animportant exogenous gene ininsect-resistant transgeniccrops，playsa crucial role in crop pest control.The cultivation and promotion of Bt transgenic crops have greatly reduced the use of synthetic insecticides and brought huge benefits to farmers.However， the safety of Bt transgenic crops has gradually atracted people'sattention.Therefore，itisofgreatsignificance todevelopasimpleandsensitivemethodfordetectionof Bt transgene in crops.This study constructs a dual-modified triple helix DNA fluorescent molecularswitch （DTHFMS）and systematicall investigates the influencing factors and variation rules of the stabilityof DTHFMS.Theresults show hat DTHFMSwithalternatingtripletsofT-AoTandC-GoCdemonstrates properstabilitymakingitsuitableforhighlysensitive detection of Bt transgenes in maize seeds.This study achievesexcellent detection results by optimizing key detection parameters. The limit of detection is 0.05pM ，with a linear range of （0.1-6.0×104）pM ：

Keywords：Bacillus thuringiensis; triple helixDNA;molecular switch; highly sensitive detection

苏云金芽孢杆菌（Bacillusthuringiensis，简称 Bt） 是抗虫类转基因作物的重要外源基因来源，对作物防治虫害至关重要。（剩余10952字）