斑马鱼模型在发育毒理学研究中的应用

关键词斑马鱼；毒性作用;安全性评价;发育毒理学中图分类号 R114;Q95-33 文献标识码ADOI号 10.16377/j.cnki.issn1007-7731.2025.24.012文章编号 1007-7731(2025)24-0053-04

Theapplicationof Danio reriomodel in developmental toxicologyresearch

KANG Guiying

(College of Animal Scienceand Technology,Inner Mongolia Minzu University,Tongliao O28OOo, China)

AbstractDanio rerio exhibits characteristics such as rapiddevelopment,external fertilization,strong reproductive capacity,and overalltransparencyduring earlydevelopmental stages,making them widelyusedas model organisms in developmentaltoxicologyresearch.TheapplicationofDanio rerio inthe safetybioactivityof traditional Chinese medicines (TCMs)andthetoxicityassessmentof environmentalpolltants weresummarizedfrom3dimensions:cardiacdevelopmental toxicity,neurodevelopmentaloxicityandvascularandbloodcelldvelopmentaltoxicityIersofrdiacevelotal toxicity research,atspecific doses,traditional Chinese mediciecomponentssuchastotal platycodinandbrucinecan induce bradycardiainzebrafish,accompaniedbysymptomsincludingcardiacarrstandhemorrhage.Environmentalpollutats like polybrominateddiphenylethers,polychlorinated biphenyls,andperfluorooctanoicacidcan triggercardiac oxidativestress in zebrafish,leading tocardiacdevelopmentaldefects.Regardingneurodevelopmentaltoxicityatspecificdoses,substances such ascadmiumchloride and bisphenolFexert neurotoxicitybyinterfering withthe expresionofkey genes involved in neural development.Regarding vascularand bloodcelldevelopmental toxicitystudies,relevantresearch hasshownthatsome TCM extracts can inhibit (e.g., methanol extractof Xihuang Wan)or promote (e.g.,total saponins from Panax notoginseng roots andflowers)Danio rerio angiogenesis,whileothers havenosignificant impact (e.g.,waterdecoctionof Geko gecko). Environmental polltants such as mycophenolic acid (MPA)can inhibit Danio rerio vascular development byregulating specific receptors (e.g.,AHR)or through direct effects.This review providesa reference forthe subsequentresearch and practical application of Danio rerio.

Keywords Danio rerio; toxic effects; safety evaluation; development toxicology

斑马鱼(Danioreri)是鲤形目鲤科鮐属的一种热带淡水鱼类，因其个体小，饲养所需空间少，养殖成本低，对水质的要求不高，且基因组与哺乳动物的基因组有高度的相似性，已成为广受欢迎的脊椎动物发育生物学模型[1-2]。（剩余7701字）