不同干燥处理的迷迭香茎叶上、中层迷迭香酸含量测定

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Determination of rosmarinic acid content in upper and middle layers of Rosmarinus officinalis L. stems and leaves under different drying treatments

WANG YuanliPEI Yunyi GUHong XU Yanfei GONG Yongwei （YuxiAgricultural Vocational TechnicalCollege，Yuxi 6531O6，China）

AbstractTocompare theefectsof differentstem/leaf positions （upper layerA，middlelayerA2），differentdrying methods （natural drying Bl， oven drying ，and different materials （leaf C1，stem C2）on the rosmarinic acid contentin rosemary，the upper and middle layers of rosemarystems and leaves were usedas raw materials.Rosmarinic acid was extracted using 70% ethanol with ultrasonic assistance，and a randomized experimental design was employed incombination with HPLC to determine therosmarinicacid content.Theresultsshowed thattherelativestandard deviation （RSD） calculated from the peak areas was 0.47% ，indicating that the established analytical method had a relatively high accuracy. The content of rosmarinic acid in A1B，C2，A2B，C2，AB，C and A treatment was 0.066 2， 0.067 9， 0.128 9 and 0.129 7 mg/mL， respectively. The rosmarinic acid contents of ， ， and treatments were O.0521，0.0573，0.1202and0.1194 mg/mL，respectively.Theresults indicated thattherosmarinic acid content extracted from stems and leaves treated with natural drying was higher than that from oven drying. The rosmarinicacid content in leaves was consistently higher than thatin stems，and among the leaves，the middle layer showed higher content than the upper layer.

KeywordsRosmarinus oficinalis L.； rosmarinic acid； ultrasonic extraction； high performance liquid chromatography

迷迭香（RosmarinusofficinalisL.）属唇形科迷迭香属芳香型多年生常绿灌木植物，为药食两用植物，可作为园林植物和经济作物进行栽培[1-2]。（剩余3855字）