烟草根黑腐病菌生防菌BM-03的防效及其发酵特性分析

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Screening and fermentation characteristics of biocontrol bacterium BM-O3 antagonistic to Fusariumsolani

LI Xiang1HAO Haohao1WU Junlin1XU Xiaojing1ZHAO Jiajia1SUNHuizhong2 （1Zhumadian Branch of Henan Provincial Tobacco Company，Zhumadian 463ooo， China； 2College of Agriculture，Henan University of Science and Technology，Luoyang 471OO3， China）

AbstractUsing plate isolation and antagonism assay techniques，a strain （designated as BM-O3）antagonistic to Fusarium solani （causalagent of tobacco root blackrot）was isolated fromtobacco rhizosphere soil.Its biocontrol effcacy wasvalidatedthrough potexperiments，and its fermentationcharacteristics weredetermined.ResultsshowedthatBM-03 exhibited an inhibition rate of 52.78% against F .solani in plate antagonism tests，while pot experiments demonstrated a biocontrol efficacy of 78.03% against root black rot. Further fermentation characterization revealed that inorganic salts had minimal impact onBM-O3’s fermentation，whereas soluble starch，yeast extract powder，and soybean meal were identified as optimal nutrients for itsgrowthand fermentation.The optimized fermentation medium wereas follws： soluble starch 30g ， soybean meal 6 g， yeast extract powder 4 g， NaCl 0.5g MgSO ·7H 2 0 0.5 g， FeSO ： ，finally adjusted to 1L with pH 8.0， 7% inoculation volume， 100mL medium per flask，and cultivation at This study provides foundational data forthe development and application of BM-O3 asa biocontrol agent.

Keywordstobacco；biocontrol bacteria； screening； control efciency；fermentation characteristics

烟草根黑腐病是一种烟草真菌病害，在烟草种植区域发生较普遍，且发生程度不一。（剩余5358字）