5种澳洲坚果种质材料叶片对低温胁迫的生理响应

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中图分类号：S664 文献标志码：A 文章编号：1003—8981（2025）03—0028—09

Abstract：【Objective】Inorder to explore the response and tolerance of diferent macadamia germplasm to low temperature stress，itprovidedatheoretical basisforbreeding localmacadamia germplasmwithwideadaptabilityand strong cold tolerance in Guizhou.【Method】The leaves of 5 macadamia germplasm materials were treated at -4∘C inan incubatorwiththecontrolofO.CandGR1，and10physiologicalindexesofcoldtolerance weredeterminedtoanalyze theresponseofleavesofdiferentMacadamiagermplasmmaterials tolowtemperaturestre，andthecoldhadinesof thefive macadamia germplasmmaterials was comprehensivelyevaluatedbythe methodofthe afliation function.【Result】Under lowtemperature stressthe physiological indexes of different macadamia materialschanged significantly.Thecontentsof MDA，proline，soluble sugar，soluble protein，relative electricalconductivityand theactivitiesofSOD，POD，CATadPO ofeachmaterialincreasedsignifcantlyandtherelativewatercontentdecreased.Withtheprolongationoflowtemperature stress，there were diferences in thecoldhardinessofeach material.QAlwasbetter thanO.CandGR1 inMDA，proline， soluble protein，andbetter thanGR1 inrelativeelectrical conductivity.QA2 was beterthan O.Cand GR1inMDA，POD In MDA，PODand PPO，QA2 performed beter than O.C，better thanGR1 in MDA，relative electrical conductivity，POD， CAT，PPO and soluble protein，and comparable to GR1 and O.C in Pro. QA3 performed beter than O.C in MDA and SOD，and beter than GR1in SOD，soluble proteinrelative electricalconductivityandrelative watercontent. Inaddition， compared with QA1，QA2 and QA3 showed morecomprehensive cold tolerance.【Conclusion】Under low temperature stress，Qian'ao seriesofmacadamianuts respondto low temperature stress through diferentphysiologicalregulation mechanisms to improvetheircold tolerance.Bymembership functionanalysis，theorderofcold toleranceof5Macadamia nut germplasm was O.C， GR1， QA3， QA2，QA1.

Keywords：Macadamia integrifolia;lowtemperature stress;physiological response;cold tolerance;comprehensive evaluation

澳洲坚果Macadamiaintegrifolia原产于澳大利亚，为多年生常绿乔木，其树体适宜的生长温度为 20～25∘C 。（剩余17317字）