GETVE1蛋白单克隆抗体制备及抗原表位鉴定

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中图分类号：S852.4 文献标志码：A 文章编号：1671-4652（2025）01-0044-08

ABSTRACT：In order to preparea monoclonal antibody against theEl protein of Getah virus（GETV），the hydrophilic region fragment （amino acid 31-182 region） of GETV El gene was constructed into pCold-TF prokaryotic expression vector in this study.After expresion and purification，TF-El recombinant protein was prepared，and mice were mmunized with it as animmunogen.With the helpof hybridoma technology，one monoclonal antibodyagainst GETVEl proteinwas successfullyobtained.The biological characterizationof theobtainedElmonoclonal antibodyshowed thatthe monoclonal antibody against E1 protein prepared in this study was suitable for indirect immunofluorescence and Western blot characterization.Meanwhile，by truncating the expression of El protein，the antigenic epitope recognized by the E1 protein monoclonal antibody was further clarified to be 73RPDYQC78 ，and the comparison of this antigenic epitope with the sequence of GETV strains that had been uploaded in GenBank revealed that this sequence Was highlyconserved among GETV strains.In conclusion，the successul preparation of monoclonal antibody to GETVEl protein provides an immunological tool for the study of GETV.

KEY WORDS： Getah virus; El protein;monoclonal antibody;antigenic epitope

盖塔病毒（Getah virus，GETV）作为甲病毒属的成员，是一种由蚊虫传播的RNA病毒，该病毒于1955 年在马来西亚首次被分离获得，随后，日本、越南、中国、澳大利亚等多个国家均报道了GETV的发生[]。（剩余10300字）