基于BSA-seq和GWAS技术的豇豆花色基因定位分析

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Abstract：Cowpea （Vigna unguiculata） is one of the major legume crops，widely distributed in tropical and subtropical regions of the world，and grown throughout China. Cowpea flower color is an important agronomic trait that plays an important role in its reproduction，but themolecular genetic basisof cowpea flower color variation is not known.To this end，this study used a combination of BSA-seq analysis based on flower color in a population of recombinant inbred lines and genome-wide association analysis of flower color in a natural population of cowpea （271 accessions），which located the gene controlling flower color between 31.9Mb and 32.3Mb on chromosome 9（ 0.4Mb ）.Theanalysis showed that this region contains30 genes，amongwhich the TRANSPARENT TESTA GLABRA 1 （TTG1） gene is located near the peak of SNP-index and is involved in the regulation of anthocyanin biosynthesis inArabidopsis.Further analysis using RT-qPCR revealed that there Was a significant difference in gene expression of TTG1 between purple and white flag petals. In addition，two pairs of polymorphic SSR primers were screened within this region and were able to distinguish between purple and white flag petal individuals in the RILs population.The results of this studycan provide some theoretical basis for cowpea flower color genetic variation and molecular breeding.

Key words： cowpea; flower color;BSA-seq; genome-wide association analysis;molecular marker

豇豆［Vignaunguiculata（L.）Walp.]是世界范围内重要的豆科作物之一，起源于非洲，广泛分布于热带和亚热带地区[1]。（剩余20846字）