菜豆响应盐胁迫的转录组分析

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Abstract： Common bean is an important edible legume in China，with rich nutritional components and extensive applications.However，its growth and development are significantly impaired bysalt stress.In this study，we investigated the transcriptional reprogramming of the seedling roots of the common bean variety F0006591 to salt stress treatment （ 0.2mol/LNaCl ）at five time points （0， 6， 9， 12 ，and 24h ）using RNAseq. If compared with the control group（ （0h） ，the transcriptional profiles under different salt stress treatments changed significantly，and a total of 3383 differentially expressed genes（DEGs）were identified. GO and KEGG enrichment analyses revealed that these DEGs are primarily involved in biological processes such as salt stressresponse，oxidative stress，and cellular defense，particularly within various metabolic pathways including plant hormone signaling，glutathione metabolism，and glycolysis. Co-expression network analysis of all up-regulated genes revealed a significantly correlated regulatory network，with abscisicacid signaling components and genes associating with antioxidant stress response and energy metabolism.RT-qPCR verified that the dynamic transcriptional profiles of 4 hormone signaling pathway-related genes，6 energy metabolismrelated genesand2 antioxidant-related genes，aligning with the results of RNA-seq analysis.Our results highlight the ABA signaling pathway with important role in coordinating the salt stress regulation in common bean.This study provides a reference for the investigation of the salt tolerance molecular mechanism in common bean.

Key words： common bean; salt stress; transcriptome analysis; differentially expressed genes （DEGs）

近年来，受自然及人为因素的影响，土壤盐渍化日益加剧。（剩余21879字）