普通细菌性疫病病原侵染普通菜豆早期的免疫反应研究

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Abstract：Common bacterial blight of common bean is one of the most serious diseases aecting the production of common bean，which occurs throughout the whole growth period of common bean，and leads to be ashigh as 80% yield loss.The most effective way to control the disease is to plant resistant varieties.In order to excavate disease-resistant germplasm，28O resources of common bean were identified for resistance to common bacterial blight，andl resistant，90 moderately resistant，and 189 susceptible materials were identified. We selected resistant Zihuayundou and susceptible Talanghuacaidou for the study of immune response in the early stage of pathogen infection.The results showed that H2O2 content of resistant material was always higher than that of susceptible material after inoculation，and the diference between resistant and susceptible materials was very significant from 12h to 96h . The results of DAB staining also showed that H2O2 accumulation existed in resistant materials at the site of pathogen inoculation.Trypan Blue staining and callose content determination were carriedout for resistant and susceptible materials after inoculation.It was found that the callse content in resistant material was significantly up-regulated 6h after inoculation. Trypan blue staining results indicated that resistant material could limit the spread of pathogens by controlling cell death at the inoculation site.These results showed that the resistant variety triggered the immune responseat the early stage of the pathogen infestation，and thus increased the resistance of the plants to common bacterial blight.

Key words： common bean; common bacterial blight;immune reaction ;cell death;callose deposition

普通菜豆（PhaseolusvulgarisL.）又名芸豆、四季豆，其籽粒富含蛋白质、膳食纤维等营养元素，且脂肪含量低，利于人体吸收，在保障营养需求和调节膳食结构方面发挥着重要作用[1-2]。（剩余14344字）