绿豆雄性不育突变体ms2的表型鉴定与基因精细定位

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Abstract：Low yield per unit is a disadvantage character of mung bean industry in China.The identification of male sterility lines is crucial for harnessing heterosis，whichcan substantially increase yield.In this study，we identified a male sterile mutant ms2 （male sterile 2） from the EMS mutant library of Zhonglv5（ZL5）.During the vegetative growth stage，ms2 exhibited no significant phenotypic diferences compared to the wild-type ZL5. In the mature period，although the floral organs of ms2 developed normally，the stamen abortion resulted in a stay-green phenotype of ms2.Genetic analysis revealed that the sterilityof ms2 is controlled bya single recessive nuclear gene. The F2 population derived from the cross between ms2 and Wankelv3（WK3）was used as the mapping population. MS2 was mapped within a 66.7kb interval flanked by markers M and M8 on chromosome 6，containing six annotated genes.Sequence analysis of these genes revealed two base transitions in the second and third introns of the glutamine synthetase gene（Vradi06g05640）in ms2.Expresson analysis indicated that theexpression level of this gene in ms2 was significantly lower than in ZL5，suggesting that Vradi06g05640 is likely the candidate gene for ms2.In summary，our study provides valuable insights into the mechanism of male sterility and the utilization of heterosis in mung bean.

Key words： mung bean;male sterile;ms2;genetic analysis ;gene mapping

绿豆［Vignaradiata（L.）Wilczek]属于豆科（Leguminosae）蝶形花亚科（Papilionoideae）豇豆属（Vigna），闭花授粉双子叶作物，是我国重要的食用豆类之一。（剩余17600字）