基于BSA-seq技术定位绿豆种皮光泽基因

打开文本图片集

Abstract：Seed coat luster not only afects the appearance quality and commodity value of mung bean，but also is closely related to their stress resistance and other traits. In this study，the F2 segregating population was constructed by crossing the shine mung bean variety Binglv15and the dull mung bean variety Jilv 20.The BSA-seq technology was used to map the mung bean seed coat luster-related genes.The results showed that the genes related to seed coat luster were mapped in the region of 48061-11475465 bp on chromosome 11，with a size of 11.43Mb . A total of 1292 genes were annotated within the candidate region，among which 163 non-synonymous mutant genes and 48 frameshift mutant genes were annotated between the parents.To further fine mapping the candidate region，eight KASP markers and seven InDel markers were developed to narow the candidate region of seed coat luster to 0.39Mb （9969581-10362350 bp）. Through the analysis of gene function annotation in the database，nine non-synonymous mutation genes were annotated in the mapping interval. Combined with qRTPCR expression analysis，six candidate genes of mung bean seed coat luster traits were screened out，including jg34407，jg34408， jg34414， jg34422， jg34433 and jg34438. jg34407 was annotated as an E3 ubiquitin protein ligase，and jg34438 was annotated as a transcription factor MYC2. It is speculated that jg34407 and jg34438 may indirectly affect the seed coatluster byregulating proteindegradationand the synthesis of secondary metabolites This study provides a theoretical basis for later gene cloning and molecular marker-assisted breeding.

Key words： mung bean;seed coat luster;BSA-seq;gene mapping;molecular markers

绿豆［Vignaradiata（L.）Wilczek]是豆科（Leguminosae）菜豆族（Phaseoleae）豇豆属（Vigna）作物[1。（剩余15923字）