Bt Cry毒素广谱检测的胶体金侧流免疫层析试纸研究与设计

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中图分类号：TS207.5 文献标识码：A 文章编号： 1000-4440（2025）10-2057-11

Abstract： BtCry toxins are important objects of environmental safety supervision of genetically modified agricultural productsand theirproducingareas.Inorderto realize the rapiddetection of Bt Cry toxins in genetically modified agricultural products，this study was conducted based on antibody-based colloidal gold lateral flowimmunochromatography，usingthe monoclonal antibodywith broad-spectrumrecognition abilityof Bt Cry toxins obtained in the previous preparation as the detection antibody，and labelingitwith colloidal goldtoforma gold-labeledprobe.Atthesame time，thepolyclonalantibodywithbroad-spectrumrecognitionabilityofBtCrytoxinsobtainedintheprevious preparation wasusedasthecapture antibodyonthe test line（Tline），andthecommercial sheepanti-mouse monoclonal antibodywasusedasthequalitycontrolantibodyonthequalitycontrolline（Cline）.Then，thecoloidalgold immunochromatographic test strip wasconstructed basedon nitrocellose membrane and other components，and its detection performance wastested.Theresultsshowed thatitheminimumvisual detection linesofthecoloidalgold immunochromatographic test strips for Cry1Ab，Cry1Ac，Cry1Ah，Cry1B，Cry1C and Cry1F were 0.5μg/mL ，0.1 μg/mL ， 0.5μg/mL ， 1.0μg/mL ， 0.5μg/mL and 0.5μg/mL ，respectively. These results indicate that the test strips developedinthis studycanbeused forbroad-spectrum rapid detectionof BtCry toxinsand have highapplication value.

Key words：Bt Cry toxins；test strip； lateral flow immunoassay；antibody；broad-spectrum detection

Bt Cry 毒素是苏云金芽孢杆菌（Bacillus thuring-iensis，Bt）在生活史后期产孢过程中代谢分泌的一类具有特异性毒杀昆虫活性的大分子晶体蛋白，其主要以生物制剂或转基因抗虫作物的形式应用于常见农林害虫（如棉铃虫、小菜蛾、褐天牛、山杨叶甲等）以及卫生媒介蚊虫（如埃及伊蚊、白纹伊蚊、致倦库蚊等）的绿色生物防治[1]。（剩余16868字）