大豆TOR家族全基因组鉴定及表达特征分析

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中图分类号：S565.1 文献标识码：A 文章编号：1000-4440（2025）10-1886-13

Abstract：The target of rapamycin（TOR）protein serves asa central regulator in plant growth，development，and stress responses.Current research on plant TOR genes remains limited，with particularly scarce investigations in important crops like soybean. This study employed bioinformatics approaches to identify the soybean TOR gene family and analyzed various characteristics including protein physicochemical properties，gene structure，protein secondarystructure，phosphorylation sites，chromosomal localization，phylogeneticelationships，evolutionaryconstraints，promoterelements，proteininteractionnetworks，andexpression patterns.Results revealed that both GmTORl and GmTOR2 proteins contain 2468aminoacids，with secondary structures predominantly composed of α -helicesand randomcoils，exhibiting193 and 191 high-potential phosphorylation sites，respectively.GmTORland GmTOR2arelocatedonchromosomes1and11，respectively.Promoterregions containresponseelements for abscisic acid（ABA），methyl jasmonate（MeJA），ethylene（ETH），gibberellin（GA），andsalicylic acid（SA）， suggesting GmTORl and GmTOR2 may participate in hormone regulation and responses to drought stress，salt stress，coldstress，andPhytophthorasojaeinfection.TranscriptomicdataanalysisdemonstratedthatGmTORlandGmTOR2 exhibitedpredominantexpressioninshoot apices，flowers，lateral buds，andpods.Underfloodingstress，theirexpression inroots decreased initiallyand then increased，whereas theoppositepatern wasobserved intheleaves.GmTORland GmTOR2 weredown-regulated inresponse tocoldstress，Phytophthorasojae infection，andethylene induction.qRT-PCRvalidation results indicated that the expresson paterns of GmTORl and GmTOR2undersalt stress were largely consistent with the transcriptome sequencing results. Here，we systematically analyzed the sequence characteristics，evolutionary relationships，and expresion paterns ofGmTORl and GmTOR2.These findings enrich our understanding of the soybean TOR gene family and provide a solid theoretical foundation for their subsequent functional studies.

Keywords：soybean；GmTORl；GmTOR2；bioinformatics；evolution；expression characteristics

大豆是中国重要的油料和粮食作物[1-2]，在农业生产过程中，常面临多种生物和非生物胁迫，进而影响其产量和品质[3-4]。（剩余19825字）