核型多角体病毒SfMNPY-Yz01对草地贪夜蛾解毒酶与防御酶活性的影响

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中图分类号：S433.4 文献标识码：A 文章编号：1000-4440（2025）10-1920-06

Abstract：Inthisstudy，larvaeof Spodopterafrugiperda were infectedwiththe SfMNPV-YzlstrainofSpodoptera fru giperdanucleopolyhedrovirus.The dynamicchanges inthe activitiesof six keydetoxifying anddefensiveenzymes inthelarvae during the infection process were systematically determined，including phenol oxidase （PO）， glutathione S-transferase （GST），carboxylesterase（CarE），catalase（CAT），peroxidase（ PD ），and superoxide dismutase （ SOD ）. The results showed that during the infectionperiod，theactivity of PO （204号 fluctuateddrastically，whichmightbeassociated with the virusregulating the melanization response in insects.Glutathione S-transferase （ GST ）maintainedhighactivityconsistently，suggestingitsroleinalleviatingoxidativestress and metabolizing toxic substances.Inthe late stage ofinfection，theactivitiesofcatalase（ CAT ）andperoxidase （POD）increased significantly，indicatingtheir involve mentin scavenging excessve reactive oxygen species（ROS）to maintaincellular homeostasis.This study clarifies the multi-levelphysiologicalresponsesofSpodopterafrugiperdalarvaetobaculovirusinfectionfromtheperspectiveofenzyme activity，providingatheoretical basis for thebiological control of Spodoptera frugiperda.

Key words： Spodoptera frugiperda； nucleopolyhedrovirus；detoxifying enzymes；defensive enzymes

草地贪夜蛾（Spodoptera frugiperda）是一种原产于美洲热带和亚热带地区的鳞翅目夜蛾科害虫，具有寄主范围广、迁飞能力强、繁殖速度快及防控难度大等特点，被国际农业和生物科学中心（CABI）列为世界十大植物害虫之一[1]。（剩余11048字）