小麦TGA转录因子家族全基因组鉴定及其响应非生物胁迫的表达分析

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中图分类号：S512.101 文献标识码：A 文章编号： 1000-4440（2025）10-1873-13

Abstract：TGA transcription factors belong to subgroupDof thebasic leucine zipper（bZIP）familyandare widely involvedinvariousbiological processesandsignal transductionpathways，playingimportantrolesinplantdefenseagainst pathogens，plantgrowthanddevelopment，andresponsestodiferentabioticstreses.Inthisstudy，weidentifiedthewheat TGA transeription factor familyusing whole-genomedataof wheat andanalyzed various bioinformatics characteristics，includinggene structures，conserveddomains，cis-acting elements，andphlogeneticrelationships.Additionall，weexploredthe expression patterns of somewheat TGA transcription factor genes in different tissues and under variousstress treatmentsusingquantitativereal-timePCR（qRT-PCR）. Atotalof 38 TaTGA transcription factor geneswere identified，which were unevenly distributed across the 21 chromosomes of wheat.Phylogenetic analysis classified these TaTGAtranscription factors into five subfamilies.Within each subfamily，the gene structures， conserved motifs，andconserveddomainsof theTaTGAtranscriptionfactors wereessentiallconsistent.Theanalysisofcis-actingelementsrevealedthatTaTGA genescontainvarious typesofcis-actingelements，coveringfunctionalregionsrelatedtoplantgrowthand development，responses toabiotic stresses，and hormoneactions.TheqRT-PCRresults indicated thattherelativeexpressionlevels of TaTGA genes werediferentiallaffected byabiotic stressesand hormonetreatments.Thefindingsof this study provide valuablecandidate genes formolecularbreedingof stress-resistant wheat andlayafoundationforfurtherresearchon the functions of TGA transcription factors in plants.

Key words： wheat；TGA；transcription factors；bioinformatics；expression analysis

自然环境中的植物不断受到各类生物和环境压力，它们对非生物胁迫、生物胁迫的响应涉及分子、细胞、生化和生理水平的变化，因此植物需要通过各种复杂的防御机制、信号网络快速响应环境因子和激素的变化，并调控相关基因的表达，以维持自身的稳态[1-2]。（剩余16054字）