辣椒胶孢炭疽菌中糖苷水解酶蛋白的找寻及生物信息学分析

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中图分类号：S436.418.11 文献标识码：A 文章编号： 1000-4440（2025）10-1926-10

Abstract：Pepper anthracnose，caused by Coletotrichum gloeosporioides，is a major disease that restricts the highquality development of thepepper industry.Clarifying the characteristicsand functionsof glycoside hydrolases（ GHs ）in thispathogenisofgreatsignificancefordecipheringitspathogenicmechanism.Inthisstudy，bioinformaticmethodswere usedto predict the GH proteins in Coletotrichum gloeosporioides isolated from peppr.Online tools including ProtParam， ProtScale，and SMARTwere employed toanalyze the physicochemical properties，hydrophobicity，and conserved domains ofthese proteins. MEGA 11 software was utilized to construct aphylogenetictreeforanalyzinggenetic relationships，while the EggNOG-mappertool wasapplied for functional annotation of orthologous group（COG） proteins.The results showed that a total of 24 GH proteins werepredicted in this pathogen.Most of these proteins wereacidic，stable，and hydrophobic，with amino acid lengths rangingfrom2O1 aa to 4OO aa.Moreover，glycoside hydrolases containing conserved GHdomains weremainlyclasified into the GH28 subfamily.Basedonthe phylogenetic tree，the24glycosidehydrolaseswereclasifiedintothreemajorcategories.COGfunctionalannotationindicatedthatthese enzymeswereprimarilyinvolvedin thetransportand metabolismofcarbohydrates，andalsoplayedrolesinbiological processes such as post-translational modification of proteinsandchaperone protein regulation.This study clarifies the number，physicochemicalproperties，andfunctionallocalizationof glycosidehydrolases inColetotrichumgloeosporiodesofpepper.It provides important theoretical supportforin-depth explorationofthemolecularpathogenicmechanismof thispathogen，accurateidentificationof targets forpathogencontrol，anddevelopmentof novelbiologicalcontroltechnologies.Meanwhile，it holds high practical value for ensuring the safe production of the pepper industry.

Key Words： pepper;Colletotrichum gloeosporioides； secreted protein；glycoside hydrolase

辣椒（Capsicumannuun）原产于南美洲[1]，既是助农增收的重要蔬菜，其提取物还可应用于工业生产，同时因富含多种营养成分，也被广泛用于医药健康产业。（剩余14692字）