短短芽孢杆菌Rh47代谢物对盐胁迫下黄瓜幼苗离子平衡和营养吸收的影响

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中图分类号：S642.2 文献标识码：A 文章编号： 1000-4440（2025）10-2047-1（

Abstract：Salt stressisamajor abiotic stress factor affecting cucumber production. Numerous studies have shown that certain plant growth-promoting rhizobacteria （PGPR）can enhance plant salt tolerance，but whether their metabolites can alleviate salt stress in plants requires further investigation.This study used cucumber‘Jinyou 401’and Brevibacillus brevis Rh47 as test materials.Seeds weresoaked in solutions containing O（control）， 25% ， 50% ， 75% ，and10O%Rh47metabolites，followedbysalt treatments withOmmol/L，25mmol/L，50mmol/L，and75mmol/LNaClsolutions toinvestigatetheeffectsofdifferent metabolite concentrations oncucumber seed germinationunder salt stress.Under75 mmol/LNaCl stress，cucumber seedlings were irigated withdifferentconcentrationsofRh47metabolitestoexaminetheireffectsonseedlinggrowth，photosynthetic parameters，the contents of K+ ， Na+ ， Ca2+ and absorption of nutrient elements.Results demonstrated that under 75 mmol/L NaCl treatment，the vigor index and total root length of cucumber seeds treated with 50% concentration of Brevibacillus brevis Rh47 metabolites were significantly higher than those in other concentration treatments（ P<0.05 ）.At the seedling stage，when the metabolite concentration was 75% ，cucumber seedlings subjected to salt stress exhibited significant increases （ P<0.05 ）in plant height，leaf area，root length，root surface area，and root volume compared to the control group.Leaf net photosynthetic rate，stomatal conductance，and transpiration rate increased by 49 % ， 59% ，and 44% respectively versus control. Shoot K+ content significantly increased（ P<0.05 ），while whole-plant Na+ content significantly decreased （ P<0.05 ）.Additionally，the 75% concentration of Brevibacillus brevis Rh47 metabolites significantly promoted the absorption of nutrient elements such as Zn ，Fe，S，and P incucumber seedlings. In conclusion，Brevibacillus brevis Rh47 metabolites at appropriate concentrations can improve the salt tolerance of cucumberbyregulating ionic homeostasis， improving nutrient uptake，and enhancing photosynthetic efficiency.

Key Words：Brevibacillus brevis Rh47 metabolites；cucumber；salt stress；ionic homeostasis；nutrient uptake

黄瓜（CucumissativusL.）是葫芦科黄瓜属一年生攀缘植物，具有产量高、经济效益好等特点，是重要的经济作物之一，在世界范围内被广泛栽培[1]。（剩余15595字）