液相色谱-质谱法灵敏高效测定细菌内源多种N-酰基高丝氨酸内酯

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关键词：N-酰基高丝氨酸内酯（AHLs）;细菌;群体感应（QS）;高效液相色谱-质谱（HPLC-MS）

中图分类号：Q936；0657.63 文献标志码：A 文章编号：1672-2043（2025）09-2478-11 doi：10.11654/jaes.2024-0903

Abstract：Inordertodevelopahigh-performance liquidchromatography-masspectrometry（HPLC-MS）methodforquantitatively detectingendogenouslowconcentrationsofN-acylhomoserinelactones（AHLs）inbacteria，thisstudyextractedAHLsproducedby bacteriathoughquidlqdetractiotiedastroetryndomatogapicoditio，ndtablshddiote withgoodlinearelatioshipallatrixect，ndigecovryate.Tultsodatteearelationoeints（f the seven AHLs were all greater than O.995，and the r valuesof all AHLs were greater than O.99o，indicating that the method hasa good linearrelationship.The substrate effectsof8 typesofAHLsare all greater than 80.0% ，andthebacterial growthmatrixhasarelatively smallimpactonthdetectionofendogenousAHLs.ThestudyalsofoundthatAHLswithlongercarbonchainshavelessinteferencein complexmatrices，ilesotaiingcaboylgrouseibitacertainsigalsuppressfctTheecoveryatesof7tysofHs all exceeded 80.0% ，indicating that this method has highrecoveryrates and goodaccuracyandreliability.The method was used todetect endogenousAHLsin bacteria，and theresults showed that C 10⋅ -HSLand C 12 -HSL were themain AHLs，furtherverifying the effectiveness of the method.Thismethodcanaccuratelydetectendogenous traceAHLsinbacteria，providingreliabletechnicalsupportforstudying endogenous AHLsin bacterial quorum sensing（QS）systems.

KeyWords：N-acylhomoserinelactones（AHLs）；bacteria;groupsensing（QS）；highperformanceliquidchromatography-ma spectrometry（HPLC-MS）

群体感应（QuorumSensing，QS）是细菌利用信号分子进行细胞间通信的重要机制，其中N-酰基高丝氨酸内酯（AHLs）是革兰氏阴性菌常见的信号分子，因此AHLs的准确检测对于理解和调控细菌的QS系统具有重要意义，这对于揭示微生物在环境治理、生物膜形成、毒力因子表达以及代谢产物生成等行为中的作用至关重要。（剩余20018字）