离线二维色谱-质谱联用技术表征槐花散抗炎活性组分的方法研究

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中图分类号：TB9 文献标志码：A 文章编号：1674-5124（2025）11-0106-12

Abstract：This study established a systematic approach for characterizing theanti-inflammatorycomponents of the classic prescription Huaihua Powder using ofline two-dimensional liquid chromatography coupled with QOrbitrap mass spectrometry （2D-LC-Q-Orbitrap MS）. First， the extract of Huaihua Powder was fractionated and enriched based on relative retention time by reversed-phase ultra-high performance liquid chromatography in the first dimension. The inhibition of nitric oxide （NO） production in LPS-induced RAW264.7 macrophages was used as an in vitro evaluation index to screen for anti-inflammatory active fractions.The results revealed thatthree fractions （A，B，and C） exhibited significant anti-inflammatory activity.Furthermore， guided by this anti-inflammatory activity，the chemical constituents of these active fractions were identified in the second dimension using UPLC-Q-Orbitrap HRMS technology with a combination of hydrophilic and reversed-phase columns.A total of 106 compounds were identified.Fraction A primarily contained amino acids，carboxylic acids，and alkaloids;Fraction B was dominated by flavonoids; and Fraction C also contained a considerable number of flavonoids.By integrating efficient separation， activity evaluation，and chemical characterization， this method enables rapid and comprehensive identification of the anti-inflammatory active components in Huaihua Powder，providing a reliable technical foundation for further research on its pharmacodynamic material basis.

Keywords： Huaihua Powder; offline two-dimensional; liquid chromatography-mass spectrometry （LC-MS）; nitric oxide （NO）; anti-inflammatory activity

0 引言

槐花散作为中医经典名方，源自《普济本事方》，国家中医药管理局亦将其列入《古代经典名方目录（第一批）》[I]。（剩余17515字）