不同标本DNA检测对疑似巨细胞病毒感染婴儿的检测效能

Efficacy of DNA Testing in Different Specimens in Infants with Suspected Cytomegalovirus Infection/ ZHANG Hua，GUO Hui，XUE Ting.//Medical Innovation ofChina，2025，22（20）： 145-148

[Abstract]Objective：To investigate the efficacy of DNA testing in different specimens for infants with suspected cytomegalovirus （CMV） infection.Method： Atotal of 10O cases of suspected CMV-infected infants admited tothe Maternaland Child Health Hospitalof Ji'an from January2021 to December 2O22 wereselected as the study subjects，and ethylene diamine tetraaceticacid-anticoagulated blood，urine and sputum samples were collected from the infants，andreal-time fluorescencequantitative polymerase chain reaction method was applied to perform CMV-DNA detection，the positive rates of different specimens，the positive rates of CMV-DNA detection and the viral loads of different specimens with positive CMV-DNA detection were compared.Result： Among the 100 children with suspected of CMV infection，there was a statistically significant diference in the positive rate of different specimens （P<0.05） ; and the positive rate of CMV-DNA in urine and sputum specimenswere higher than thatin blood specimens， the differences were statistically significant （ P <0.05）. There was statistically significant difference in the viral load of positive CMV-DNA tests in blood， urine and sputum samples （ P <0.05）; the viral load of positive CMV-DNA tests in sputum was higher than those in blood and urine，the differences were statistically significant ⋅P<0.05， ; and the viral load of positive CMV-DNA tests in urine was higher than that in blood， the difference was statistically significant （P<0.05） . Conclusion： Infant sputum CMV-DNA test has high diagnostic value for CMV infection.

[Keywords] Human cytomegalovirusDNA Infant Sputum

First-author's address： Department of Pediatrics，the Maternal and Child Health Hospital of Ji'an， Ji'an343000，China

doi：10.3969/j.issn.1674-4985.2025.20.034

巨细胞病毒（CMV）是一种常见的疱疹病毒，主要通过体液传播。（剩余6415字）