基于网络药理学和分子对接的葛根素治疗视网膜静脉阻塞作用机制的研究

打开文本图片集

中图分类号：R774.1 文献标志码：A DOI：10.3969/j.issn.1003-1383.2025.07.002

【Abstract】ObjectiveTo studythekeytargetsand mechanismsof puerarininthetreatment ofretinal veinocclusion （RVO）basedonnetwork pharmacologyand moleculardocking technology.MethodsThechemical structureinformationof puerarin was adopted，and thenthetargets of puerarin weresearched through SwissTargetPredictiondatabase and SuperPred database，andRVO-related targets wereobtained through GeneCards database and OMIMdatabase.The intersectionbetween the targetsofpuerarinandtheRVO-related targets were taken，andthenthepotentialtargetsof puerarinfor treating RVO wereobtained.Protein-protein interaction（PPI）analysis wasconductedontheSTRING platform，andtheresultswere visualizedandcalculatedbyCytoscape3.1O.3software，soastoobtainthepotentialkeytargetsof puerarinfortreatingRVO. Geneontology（GO）functional enrichment and Kyoto encyclopedia of genesand genomes（KEGG）pathway analysis were performed with Metascape Version3.5 to explore the potential mechanismsbywhich puerarinactedagainstretinal veinocclusion（RVO）.Inaddition，moleculardocking technology wereemployedto validatethebinding afinities between puerarin anditskeytargets.ResultsAfterscrening，13OpotentialtargetsforpuerarininthetreatmentofRVOwereidentified， including2Opotentialkeytargets.GOfunctionalenrichmentanalysisshowedthatthesetargetsweremainlyrelatedtobiological processes such as oxidativestressresponse andcell migration，celluarcomponentssuch as extracelular matrix and plasma membrane lipidraft，kinase binding，oxidoreductaseactivityand other molecular functions.KEGG signaling pathway enrichment analysisshowedthat themainpathwaysof puerarininthetreatmentofRVO wereHIF-1 signaling pathway， MAPK pathway and AGE-RAGE signaling pathway in diabetic complications.Molecular docking showed that puerarin had strong binding activity with six targets.ConclusionPuerarin may act on targets such as PTGS2，AKT1，MAPK3，CASP3， TNF，and IL-6，and playarole in the treatment of RVObyregulating HIF-1，MAPK，AGE-RAGE signaling pathway in diabetic complications and other signaling pathways.

【Keywords】network pharmacology；puerarin；retinal vein occlusion（RVO）；molecular docking

视网膜静脉阻塞（retinalveinocclusion，RVO）是指视网膜静脉血管部分阻塞或完全阻塞，造成视网膜静脉扩张或出血的视网膜血管性疾病，是导致视力障碍甚至视力丧失的重要原因。（剩余13116字）