黑线姬鼠肠道纤维素降解菌的筛选及酶活力比较
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中图分类号：Q95 文献标识码：A DOI编码：10.3969/j.issn.1006—6500.2025.11.009
Screeningof Intestinal Cellulose-Degrading Bacteriaand Comparisonof Enzyme Activity in the Apodemus agrarius
SONG Xiaoning， SUN Xueying，LI Lu，LU Sihan， SUN Yue， JIN Zhimin （CollegeoflifeScienceandTechnology，MudanjiangNormalUniversityMudanjiang Heilongjiang157o1，China）
Abstract：Apodemusagrariusisamajorpestrodentinagricultureandforestry，inflictingconsiderabledamageoncropsandforest vegetation.Tissudyplayedavitaloleinexploreagreecotrolstrategroughteinvestigatioofintestinalcellosdecoo ing bacteriain A .agrarius，with thegoaloffacilitating ecologicalregulation.Anintestinalcelllose-degrading bacterial strainwas isolatedusingasodiumcarboxymethylcelulose（MC-Na）medium，byinoculatingdilutedcecalcontentsontoagarplatesviathestreak plate method.Theisolateswereidentifiedbasedonmorphologicalcharacteristicsand16SrRNAgenesequeneanalysis.Congored staining was employedtovisualizethehydrolysiszonesofthebacterialstrains，andthoseexhibitinglargerclearzoneswereselectedfor furtheranalysis.Endoglucanase activity （Cen），exoglucanase activity （Cex），and β -glucosidase activity （BG） were quantified using the 3，5-dinitrosalicylic acid（DNS） method.Five cellulose - degrading bacterial strains were successfully isolated from the intesties of A.agrarius，allofwhichwereBacilusvelezensisandtheHCvalueofstrainHX-cwasthelargestbytransparentcirclebservation， and it was preliminarily judged that strain HX -c had the strongest ability to degrade cellulose，and the enzyme activity of five strains was subsequently tested. Comparative analysis revealed that strain HX-d exhibited the highest endoglucanase and β -glucosidase actities，whereasHX-bshowedtheighestexoglucanaseactivityRdentgutmicrobiotaisichandhasstrongcelulosedgradingcapabilties，hichissignificantforthdgradationndutilizationofiomassprovidingaefereefotedevelopmentlicatio and optimization of enzyme production conditions of cellulose enzymes in rodent guts.
Key Words： Apodemus agrarius;intestinal cellulose-decomposing bacteria; bacillus cereus; celulose activity assay
纤维素是植物细胞壁的主要成分，由葡萄糖通过β-1，4糖苷键连接。（剩余9351字）
