铝胁迫对3种豆科牧草种子萌发 及幼苗生长和生理特性的影响

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中图分类号：S541 文献标识号：A 文章编号：1001-4942（2025）07-0083-09

AbstractIn order to understand the growth and physiological responses of three legume forage grasses （Trifolium repens L.，Medicago sativa L.，Lotus corniculatus L.）to aluminum stress，and to compare their differences in aluminum tolerance，six aluminum concentrations of 100，200，300，500，700 and 1 000mg⋅L-1 （204号 were set in this study. The seed germination，seedling growth and physiological indexes were measured，the evaluation indexes of aluminum tolerance were screened out through principal component analysis，and the aluminum tolerance was evaluated by membership function method.The results showed that low concentration aluminum stress （100 or 200mg⋅L-1 ） had a certain promoting effect on seedling growth and biomass of Tδ repens M. sativa and L .corniculatus. As the increase of aluminum concentration，the seed germination and growth indicators of the three legume forage grases significantly decreased，showing the greatest inhibition to root system.Moreover，as the increase of aluminum concentration，the contents of soluble sugar and proline as well as the activities of peroxidase（POD）and catalase（CAT） in the three legume forages showed the trend of increasing first and then decreasing，reaching their maximum values at aluminum concentration of 200 or 300mg⋅L-1 The relative root length，relative germination index，relative viability index，relative malondialdehyde content and relative peroxidase activity selected by principal component analysis could be used as evaluation indicators for aluminum tolerance of T . repens，M. sativa and L.corniculatus. The average membership function value of the fiveindicators calculated by membership function method could be used to identify the aluminum tolerant capacity，and the results showed that T . repens had the strongest aluminum tolerance， followed by M. sativa， and the aluminum tolerance of L. corniculatus was the worst. These results could provide the theoretical basis and certain reference for screening aluminum-tolerant leguminous forage germplasms and breeding new varieties in the southwest region of China.

KeywordsLegume forage grass； Aluminum stress； Seed germination; Osmoregulation substances ； An-tioxidant enzymes

铝作为地壳中含量最丰富的金属元素，其含量约占 8.3%[1] 。（剩余11220字）