高粱 GAox 基因家族的鉴定及其在种子萌发中的表达分析

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中图分类号：S514：Q781 文献标识号：A 文章编号：1001-4942（2025）07-0010-09

Identification of Sorghum GAox Gene Family and their Expression Analysis during Seed Germination

liang Yuwen，Ren Yan，Chen Manjing，Zhao Qiang，Zhang Guobing， Shen Jiaqi，Gao Xu， Shao Mingbo （ Guizhou Institute of Upland Crops， Guiyang 55OoO6，China）

AbstractThe GAox family are the key enzymes in the gibberelin synthesis pathway.This study conducted identification and bioinformatics analysis of the GAox family in sorghum （Sorghum bicolorL.）and validated the expression of GAox genes during seed germination using qRT-PCR. The results showed that a total of 16 GAox gene family members were identified，and they unevenly distributed on 6chromosomes and encoded 323 to 498 amino acids.These GAox proteins were hydrophilic proteins located in the cytoplasm with isoelectric point as 4.80 to 9.58，instability index as 36.17 to 54.87，and fat coeficient as 69.29 to 90.81. Through phylogenetic analysis，it was found that the GAox gene family could be divided into four subfamilies as GA20ox，

GA3ox，C20GA2ox and C19GA2ox，which contained 4，2，3 and 7 members，respectively. The intra- and inter-group collinearity analysis of GAox genes showed that 5 pairs of duplicated genes were identified in sorghum，and 29 pairs of colinearity genes were found between sorghum and rice. Through the analysis of gene structure and conserved motifs，it was found that the sorghum GAox gene family members contained 1 to 4 exons，O to 3 introns and 15 conserved motifs.Allthe 16 sorghum GAox members contained abscisic acid response elements， jasmonic acid methyl ester response elements and light response elements，suggesting their functions related to plant growth and development，light response and plant hormones.Further analysis of expresion patterns showed that the expression of sorghum GAox genes was tissue-specific，suggesting different functions in sorghum growth and development. The qRT-PCR analysis revealed that the 16 genes were upregulated during seed germination，but the expression peak occurred at diffrent time，which suggested that different GAox genes might play roles at different germination stages of sorghum seeds. These results could provide a theoretical basis for subsequent functional research of the sorghum GAox genes.

KeywordsSorghum bicolor L.;GAox gene family； Bioinformatics analysis; Seed germination; Expression pattern

在作物收获季节，若遇到高温高湿天气，种子休眠性常会被打破，导致水稻[1]、小麦[2]、高梁[3]和大麦[4]等作物穗发芽灾害，严重危害粮食产量和品质，全球范围内每年造成高达10亿美元的经济损失[5]。（剩余10175字）