玉米ZmRUM1基因的克隆及生物信息学分析

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中图分类号：S513：Q781 文献标识号：A 文章编号：1001-4942（2025）07-0019-07

Cloning and Bioinformatics Analysis of ZmRUM1 Gene in Maize

Wang Wenqi， Wang Panpan， Zhang Yanling， Liu Qingqing，Hong Shuangshuang， Zhao Gaopeng，Liu Hongchang，Wang Cuiling （Department of Agronomy， Henan University of Science and Technology，Luoyang 471023， China）

AbstractTo investigate the function and molecular mechanism of the AUX/IAA family gene ZmRUM1， it was cloned from maize inbred line CML288 and analyzed by bioinformatic methods in this study.The results showed that the CDS sequence of ZmRUMl gene was 810bp ，encoding 269 amino acids. The molecular formula of ZmRUM1 protein was C1241H1980N348O393S11 ， the molecular weight was ， the theoretical isoelectric point （pI）was 8.56，the instability factor was 52.41 indicating it was an unstable protein.It had the fat solubility index as 69.11，contained a structural domain unique to the AUX/IAA family，located in the nucleus，contained no transmembrane structural domain or signal peptide，and was a hydrophilic protein with the hydrophilicity coefficient as -0.399 . It was the closest relative to Sorghum bicolor and Miscanthus lutarioriparius.These results could provide the theoretical basis for in-depth study of the biological functions of ZmRUM1 gene.

KeywordsAUX/IAA; Maize; ZmRUM1 ; Bioinformatics analysis

生长素（Auxin）是首个被人类发现并深人研究的植物激素，其在植物体内广泛分布，对组织器官的形成以及果实的发育成熟等过程起到重要作用[1-2]。（剩余8264字）