君迁子DIWRKY3基因的克隆及其对逆境胁迫的响应

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中图分类号：S665.3：Q781 文献标识号：A 文章编号：1001-4942（2025）07-0026-10

Cloning and Expression Analysis of DiWRKY3 Gene in Diospyros lotus under Abiotic Stresses

Zhang Xiaona， Yan Aoyang，Suo Dekun， Zhou Ruijin，Hou Xiaojin，Hu Huiling （College of HorticultureandLandscape Architecture，Henan Instituteof Science & Technology，Xinxiang 4530oo，China）

AbstractIn order to study the specific role of DlWRKY3 gene in response to abiotic stresses，the nucleotide sequence of DlWRKY3 gene was cloned using homologous cloning method with the nucleotide squence of DkWRKY3 gene of Diospyros kaki as reference.Bioinformatics and expresson analyses of DlWRKY3 gene were performed. The results showed that the length of open reading frame （（ORF） of DlWRKY3 gene was 951bp ， encoding 316 amino acids. The molecular formula of DlWRKY3 protein was C1490H2401N447O490S15 ， and the theoretical isoelectric point was 8.52.It was an unstable alkaline hydrophilic protein with not transmembrane domain or signal peptide region. The predicted secondary structure of this protein was dominated by α -helix and random curling. It was a type II WRKY transcription factor with the typical WRKYGQK domain and a C2H2 zinc finger structure. Homologous comparison showed that this gene was closely related to Arabidopsis AtWRKY40 and coton GhWRKY3. The results of fluorescence quantitative PCR indicated that the expression of DlWRKY3 was induced by low temperature stress，and inhibited by drought and salt stresses.In this study，DlWRKY3 gene was successfully cloned，which laid a foundation for further study on the role of DlWRKY3 gene in response to abiotic stresses，and provided the important gene resource and theoretical basis for stress-resistant molecular breeding of Diospyros plants.

KeywordsDiospyros lotus L.; DlWRKY3; Gene cloning；Bioinformatics analysis； Abiotic stresses

WRKY转录因子是植物特有的一类转录因子大家族，其氨基酸序列含有一段由 WRKYGQK构成的7个氨基酸结构域和由C2H2或C2HC型锌指结构组成的约60个氨基酸的高度保守结构域[1]。（剩余14193字）