干旱胁迫下外源 H₂O₂ 对豌豆种子萌发和幼苗生理特性的影响

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关键词：干旱胁迫；过氧化氢（ H2O2 ）;豌豆；种子萌发；幼苗生理特性中图分类号：S643.3 文献标识号：A 文章编号：1001-4942（2025)07-0077-06

Effects of Exogenous H2O2 on Pea Seed Germination and Physiological Characteristics of Seedlings under Drought Stress

Wang Zhike，Li Yumei,Wang Yabo，Wu Yadi (School of Agriculture and Bioengineering， Longdong University/Gansu Key Laboratory of Protection and Utilization for Longdong Biological Resources and Ecological Restoration， Qingyang 7450oo，China)

AbstractTo clarify the mitigation effect of exogenous hydrogen peroxide ( H2O2 ） on pea seed germination and seedling growth under drought stress，the double-layer filter paper culture dish method was used in this experiment to study the effects of 0.03% ， 0.06% ， 0.09% ， 0.12% and 0.24% of H2O2 immersion on pea seed germination and seedling growth and physiological characteristics under drought stress (（ 4% PEG，P). The results showed that 4% PEG stress significantly inhibited seed germination and seedling growth of pea, and the germination potential，germination index and vigor index of the seeds as wellas bud and root length and fresh weight of seedlings alldecreased significantly，so it could beusedas the concentration to identify drought stress.The treatment of 0.03% H2O2 immersion （O.03H+P） significantly improved the germination potentialgermination index and viability index of pea seeds under drought stress，promoted the growth of seedling buds and rots，as well as improved the activities of ascorbate peroxidase （APX），superoxide dismutase (SOD) and catalase（CAT）,reduced the malonaldehyde （MDA）content，and increased the content of proline and soluble sugar in buds and roots. In general, the treatment of 0.03% （204 H2O2 immersion ( 0.03H+P ）could significantly mitigate the inhibition effects of drought stress on seed germination and seedling growth of pea，increase the activity of antioxidant enzymes，reduce the oxidative damage under drought stress，and enhance its drought resistance，but the effect of high concentration H2O2 immersion was not obvious. Therefore, 0.03% （20号 H2O2 could be used as the suitable concentration for pea germination and growth under drought condition.

KeywordsDrought stress ; Hydrogen peroxide (H202) ） ; Pea; Seed germination; Seedling physiological properties

干旱胁迫导致作物产量和质量下降，如今由于气候的变暖而更加严重[1]，需要采取措施提高作物对全球气候变暖所引起干旱的耐受性[2]干旱胁迫会引起植物许多生理生化特性的变化，这使得维持植物细胞的渗透调节至关重要[3]植物的抗旱程度随植物种类、品种、物候阶段和受胁迫的时间而变化[4]。（剩余13605字）