鲫属鱼类线粒体D-loop区结构及其系统发育分析

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中图分类号：S917.4 文献标识码：A 文章编号：1006-060X（2025）09-0077-06

Abstract：Carassus is agenus with widedistributioniAsiaandEurope.Due totheircomplex and variableapearancemophology， as wellas the existenceofmultiple ploidiesandreproductivemethods，theclasificationofthisgenus hsnotbeen wellresolved. In this study，PCR andDNA sequencing were employed tosequence the mitochondrial DNA（mtDNA）D-loopregion offour Carassius species：C.auratus auratus，C.auratus var.SuoguC.auratusvar.Pengze，and C.auratus.Theobtained sequences were then compared withthose publishedonNCBITheresultsshowedthat telengthofthe mtDNAD-loopsequences ofCarasiusrangd from 919 bp to 959 bp，with the average content of adenine （A） and thymine （T） being 65.7% and showing a bias in the base composition of A+T. The average interspecific genetic distance was between O.002 and O.O78. The genetic distance between C. auratus auratus and C.auratus var. Suogu， as well as between C.auratus var. Pengze and C.auratus gibelio clone D ，was the shortest，while thatbetween C.auratus in Qihe Riverand C carassius was the longest.A phylogenetic analysis was conducted on 15 Carassius species via the neighbor-joining method，clusteringthe15speciesintofourmajorcdes.CladeIincluded10speciesofnativespeciesandselected breeds in China. Clade II consisted of three species of Japanese crucian carp.Clade III was C. cuvieri，andCladeIV was C carassius. Clade Icould be further clasified into three subclades （A，B，and C）. C.auratus auratus showed acloser kinship with C⋅ auratus var. Suogu，while C.auratus var.Pengze had a closer kinship with C.auratus gibelio.

Keywords：Carassius;D-loop region; phylogeny

鲫属（Carassius）系鲤形目（Cyprini-formes）鲤科（Cyprinidae）鱼属，是我国重要的经济鱼类之一，广泛分布于亚欧地区。（剩余6810字）