农杆菌介导的水稻遗传转化的优化研究

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中图分类号：S511.035文献标志码：A

文章编号：1673-6737（2025）05-0018-06

Optimization of Rice Genetic Transformation Mediated by Agrobacterium Tumefaciens

ZHOU Xiao-tong （Northeast Forestry University，Harbin 15OOOO，China）

Abstract：Objective：TooptimizethegenetictransformationofricemediatedbyAgrobacterium.Method：Mature embryos ofindicaricevariety9311，Minghui-86，andjaponicaricevarietyNihon Harunawereusedasresearch materialsfor genetictransformationoptimizationofrice.TheAgrobacterium strainusedintheexperiment was LBA404，and the plasmid used waspCAMBIA1301A At MYB44.After preparing multiple solutions，prepare Agrobacterium competentcells. Transfer plasmid pCAMBIA1301A At MYB44 into Agrobacterium strain LBA4404 using prepared Agrobacterium competent cells.Applytheimportedbacterialsolutiontoinfectthreetypesofriceandachievetheirgenetictransformation.Extract DNA molecules fromrice plantsafter genetictransformationandperformPCRdetection.Result：Afteroptimization，the DNAextractionamount，DNApurity，andDNAintegritywereallhigher.Afteroptimization，thegenetictransformation efectofindicaricevarietiesisbeter.Afteroptimization，thePCRbandsareclearer，andtheocurenceofnon-specific bandsis lessobvious，resulting inahigher positiverateofPCRdetection.Conclusion：TheoptimizationofAgrobacterium mediatedrice genetic transformationhas beensuccesfullyachievedinthestudy，which hassignificantpractical significance.

Key Words：Agrobacterium;Plasmid; Rice genetic transformation;DNA molecule extraction;PCR detection

随着生物技术的不断发展，在作物新品种培育与改良中，植物遗传转化发挥着日益关键的作用，其中农杆菌介导法作为经典且高效的植物遗传转化手段，在水稻等重要粮食作物的研究中得到了广泛应用。（剩余6405字）