麦类壳多胞斑点病菌的快速检测方法研究

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摘要  利用麦类壳多胞斑点病菌β-tubulin一段保守基因序列，设计特异性引物进行麦类壳多胞斑点病菌的检测，并进行特异性与灵敏度验证。结果显示，该方法特异性强，灵敏度可达5 pg/μL，适合于麦类壳多胞斑点病菌的快速检测。

关键词  β-tubulin基因；麦类壳多胞斑点病菌；特异性；灵敏度

中图分类号  S41  文献标识码  A

文章编号  0517-6611（2024）18-0118-02

doi：10.3969/j.issn.0517-6611.2024.18.025

开放科学（资源服务）标识码（OSID）：

Study on the  Rapid Identification Method of Parastagonospora pseudonodorum

LI  Jin-qing1，2，WANG Ying-chao1，3，SHAO Xiu-ling1 et al

（1.Technical Center of Qingdao Customs，Qingdao，Shandong 266109；2.Technical Center of Yantai Customs，Yantai，Shandong 264000;3.Linyi Vocational University of Science and Technology，Linyi，Shandong 276000）

Abstract  This study utilized partly conserved gene sequence of β-tubulin in Parastagonospora pseudonodorum，designed specific primers for the detection of Parastagonospora pseudonodorum，with specificity and sensitivity verified.The results showed that this method had strong specificity and a sensitivity of up to 5 pg/μL and suitable for rapid detection of Parastagonospora pseudonodorum.

Key words  β-tubulin gene;Parastagonospora pseudonodorum;Specificity;Sensitivity

基金项目  国家重点研发计划（2021YFD1400100，2021YFD1400103）；青岛海关科研项目（QK202053，QK202016）。（剩余7305字）