响应面优化法在绣球菌液体菌种培养中的应用

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摘要 为获取生物活性高的绣球菌液体培养菌种，采用响应面优化法对液体培养条件进行了优化，以菌丝生物量为指标，建立了菌丝生物量与培养pH、温度、装瓶量的数据模型，得到了适宜的培养条件，即pH为4.5，培养温度为23.9 ℃，装液量为114 mL，在此培养条件下，菌丝生物量达5.9 g/L。

关键词 绣球菌；菌丝生物量；响应面优化法

中图分类号 S 646  文献标识码 A  文章编号 0517-6611（2023）23-0044-04

doi：10.3969/j.issn.0517-6611.2023.23.010

Application of Response Surface Optimization Method in Culture Conditions of Liquid Spawn of Sparassis crispa

HE Li-hu， WANG Wen-guang，  LI Juan-li

（Yangling Vocational & Technical College，Yangling，Shaanxi  712100）

Abstract Response surface methodology （RSM） was used to optimize the culture conditions for obtaining high bioactivity Sparassis crispa species， and mycelial biomass was taken as the index， the data model of mycelium biomass， culture pH， culture temperature and culture volume was established， and the suitable culture conditions were obtained. The culture conditions were pH 4.5， culture temperature 23.9 ℃ and culture volume 114 mL， the mycelium biomass reached 5.9 g/L.

Key words Sparassis crispa;Mycelial biomass;Response surface optimization method

基金项目 杨凌职业技术学院2021年科技创新项目（ZK21-69）；陕西省2023年农业科技创新项目。（剩余4309字）