山田胶锈菌效应蛋白GyHGSRE1的功能初探

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中图分类号：S763；Q89 文献标志码：A 文章编号：1000-2006（2025）05-0209-08

Abstract：【Objective】The studydeterminedthecore biological functionof theeffector proteinGyHGSRE1secreted by theGymnosporangium yamadae haustoria.This work provides foundational data for elucidatingthe molecular mechanisms of G yamadae effector protein.【Method】The haustorial transcriptome of G ：yamadae prioritized GyHGSRE1 asa highly expressed effector （ FPKM=∇117.92 ）.MEME（http：//meme-suite.org/） predicted its two-dimensional structure，while Tencent AI（https：//drug.ai.tencent.com）generated thethree-dimensional model.Real-time PCR quantified GyHGSRE1 expression level during fungal infection.Yeast secretion assys validated thesignal peptide'ssecretory capacity.Transient expresion via Agrobacterium tumefaciens assessed GyHGSRE1 functionin Nicotiana benthamiana and apple （Malus domestica） leaves.【Result】GyHGSRE1 contains an N-terminal serine-rich signal peptide.qRT-PCR demonstrated peak expresion during haustorium maturation and spore development （pycniosporophores/aeciospores））. The protein was localized toplantcellcytoplasmand nuclei，triggering celldeath and immune responses in leaf cels ofN.benthamiana.Ful-ength GyHGSRE1inducedcell death inapple leaves，butdeletionofthesignal peptideatenuated thisactivity.【Conclusion】Theglycine-serine-richatypical effectorGyHGSRE1exhibitsduallocalizationandcel necrosis-inducing efectsacross plant species，implying broad-spectrumelicitorpotential.Itsexpresioncorrelates with critical infectionstages（hostcolonization and sporulation）.The signalpeptide may mediatefunctional specificity，likely influencing host-pathogen recognition.

Keywords：Gymnosporangiumyamadae；atypical effctor protein；elicitor；signal peptide；plant immunity

植物与病原菌的长期互作过程中进化出两层免疫防御系统，第1层为模式触发的免疫（patterntriggeredimmunity，PTI），第2层为病原菌效应蛋白触发的免疫反应（effector-triggeredimmunity，ETI）[1]。（剩余15137字）