马尾松抗病种质资源筛选与基于液相芯片的SNP初步分析

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中图分类号：S722.5;S763 文献标志码：A 文章编号：1000-2006（2025）05-0001-10

Abstract：【Objective】Pinus massoniana（mason pine），a native conifer species endemic to southern China's mountainous regions，holds significant economicand ecological value.However，thespread of pine wodnematode disease（PWD）has necesitated breeding programs toenhance itsresistance，and to provide critical germplasm for PWD-resistantbreeding.【Method】A total of725half-sib progenies（from51families）derivedfrom PWD-resistant P masoniana treeswere collctedacross fiveprovinces（cautonomousregion））.Resistance wasassessedvia artificial inoculation with 5OOO pine wood nematodes per seedling. Genome-wide association studies （GWAS）were performed by integrating phenotypicdatafrom inoculationassays with targeted sequencingdata generatedusinga1O1.6Kliquid-phase probe panel.【Result】Four highlyresistant and tenresistant families were identifiedasvaluable germplasmresources. Statisticalanalysesrevealedthat mixed linearmodel（MLM）obtained five significant SNPs（phenotypic variance 收稿日期Received：2024-12-01 修回日期Accepted：2025-01-02 explained，PVE 7.69%-11.24% ）.General linear model（GLM）obtained four significant SNPs（total PVE 5.96% ）. BLINKmodel obtained eleven significant SNPs（PVE 12.86%-26.43% ）.Nine candidate genes were functionally annotated，including P45O familyproteins，pectin methylesterase inhibitors（PMEIs），andothers.Thesegenes likely regulate post-invasion immune responses by modulating cell wall structure and membrane proteinrecognition. 【Conclusion】Fourteen P .massoniana resistant families can serve as valuable breeding materials for disease-resistant varieties.Ninecandidatedisease-resistant genesidentifiedthrough GWASwillcontributetoelucidating thedisease resistance mechanisms of P .massoniana and facilitating early selection of disease-resistant varieties.

Keywords：masson pine（Pinus massoniana）； pine wilt disease （PWD）；genome-wide association study; resistance genes

马尾松（Pinusmassoniana）是我国的乡土树种，广泛分布于南方17个省（市、区），主要用于制浆造纸、建筑、采脂等[1]，在我国速生丰产材用和脂用林基地建设中占据极其重要的地位。（剩余19082字）