基于UPLC-Q-TOF-MS/MS技术解析温经汤基准样品成分

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中图分类号：S853.7；R284.1 文献标志码：A 文章编号：1671-4652（2025）05-0082-14

DOI：10.16872/j.cnki.1671-4652.2025.05.010

ABSTRACT：The UPLC-Q-TOF-MS/MS method was used to analyze the chemical composition Wenjing Decoction， and the separation was carred out on a Kromasil 1O0-1.8-C18 column with a gradient elution acetonitrile 0.05% formic acid water solution at a volume flow rate 0.4mL⋅min-1 and a column temperature 35°C . ESI was used with both positive and negative ionization modes，and thecomposition was identifiedand confirmed by analyzing the accurate molecular weight and the massfragmentation patternby UPLC-Q-TOF-MS/MS as wellas comparing with the multistage mass spectrometricdata from the standard substances，stware prediction andliterature data.Atotal 75 chemical components were identified from the benchmark sample（extracting solution） Wenjing Decoction，including 28 saponins，16 terpenoids，13flavonoids，5 phenolicacids，5phthalides，3ketosteroidand5othercompounds，andall thecomponents were present in relevant herbs.Inconclusion，UPLC-Q-TOF-MS/MS enables a rapid，comprehensive method for systematic qualitative analysis thechemical constituents in the extracting solution Wenjing Decoction，whichcan providea basis for clarifying the effective substances and improving the level quality control.

KEY WORDs： Wenjing Decoction; benchmark sample； chemical composition; UPLC-Q-TOF-MS/MS

古代经典名方至今仍广泛应用且疗效确切，是具有明显特色和优势的古代医籍所记载的方剂。（剩余15014字）