基于GBS技术的贵州铜仁地方茶树资源遗传关系研究

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中图分类号：S571.1； Q346+ .5;Q789 文献标志码：A 文章编号：1671-4652（2025）05-0023-08

ABSTRACT：To provide reliable evidence for originand evolution，germplasm innovation，selective breding，reasonable protection and utilization of genetic resources of local tea germplasm，genotyping by sequencing（GBS） technology was applied to identifysingle nucleotide polymorphism（SNP）loci for6O local tea germplasms resources from five populations in Tongren city of Guizhou province，and subsequently，phylogenetic trees，principal component analysis （PCA），and population structure analysis were performed to evaluate the genetic relationshipsof these local tea germplasms resources based onthe obtained SNP loci.The results showed that：After screening with parameters of minor alele frequency > 0.05 and data integrity >0.80 ，a total of 6 749 675 SNP loci were retained，and the average of integrity-ratio was 92.75% . According to the phylogenetic tree，principal component analysis and population structure analysis，samples from the same region exhibited closer genetic relationships，and 6O local tea germplasms resources were clustered into two clusters：samplesof Dejangand Yanhe wereclustered intoacluster，samplesof Yinjiang and Shiqian wereclustered into a cluster， suggesting distinct ancestral origins.

KEY WORDS： tea germplasms resources;genotyping by sequencing； SNP; genetic structure

中国西南地区是世界茶树（Camellia sinensis）原产地和起源中心，现存丰富的地方茶树资源[1-2]。（剩余15717字）