芍药花扣囊复膜酵母发酵液对 H₂O₂ 诱导的细胞衰老模型抗衰老作用

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中图分类号： S682.1+2 文献标志码：A 文章编号：1671-4652（2025）03-0085-11

ABSTRACT：This studyaimed to investigate theameliorative efects of Saccharomycopsis fibuligera-fermented Paeonia lactiflora flower extract（SF）oncelular senescenceand itsunderlying mechanisms，thereby providing a scientific foundationfor developing natural anti-aging products. SF was prepared using thefermentation of S.fibuligera.The impact of thefermentationbrothonNIH3T3cellviability was assessed using theWST-1cellviabilityassaykit.Theintracellar reactive oxygen species （ROS）levels were detected with the DCFH-DA fluorescent probe.Mitochondrial membrane potential（MMP）was measured using the rhodamine 123（RH123） fluorescent probe. Nuclear damage was evaluated by propidium iodide （PI） fluorescence staining. Cellapoptosis was analyzed using AO/EB fluorescence staining and Annexin V-PI/FITC flow cytometry.Cell cycle distribution was determined by PI single staining combined with flow cytometry.

Molecular docking was employed to study the interactions between the active components of P . lactiflora and anti-aging target proteins （SIRTl and tyrosinase）．The results showed that the SF exhibited no significant cytotoxicity to NIH3T3 cells at concentrations below 800μg⋅mL-1 . In the H2O2 -induced cellular senescence model，the fermentation broth significantly improved cell morphology，reduced intracellar ROS levels，protected mitochondrial membrane potential，decreased nuclear damage，lowered apoptosis rates，and reversed cellcycle arrst.Molecular docking results showed that benzoyloxypaeoniflorin from P . lactiflora had the strongest binding affinities with SIRTl and tyrosinase，with binding energies of -52.16 and -46.55kJ⋅mol-1 ，respectively，indicating its significant anti-aging potential. In conclusion， the SF possesss multiplerolesincell protectionand anti-aging effects，which maybe associated with the increased active components during fermentation， particularly gallic acid and benzoyloxypaeoniflorin.

KEY WORDs： Paeonia lactiflora flower； Saccaromycopsis fibuligera fermentation; anti-aging;cellular senescence model；molecular docking

随着我国人口老龄化的加剧，与衰老相关的疾病，如神经退行性疾病、恶性肿瘤和心血管疾病等发病率显著上升，成为公共卫生领域的重大挑战[1]。（剩余16453字）