一株耳廓狐源化脓隐秘杆菌的分离鉴定与耐药性分析

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中图分类号： S852.61+9 文献标识码：A文章编码：1005-8567（2025）06-0106-06

Isolation， Identification and Drug Sensitivity Test of a Trueperella pyogenes Strain from Vulpes zerda

WANGGuoqian，CHENXuanjiao，ZENGFanwen，SAJiaqi，PENG Shiming （Guangzhou Zoo&Guangzhou WildlifeResearch Center，Guangzhou Guangdong510075）

Abstract：To identifythe pathogenic bacteriacausing thedeathofa Vulpes zerda fromrespiratoryfailureatazoo inGuangdong Province，lung tissue sampleswere collcted forbacterial isolationandpurification，morphological microscopy，biochemical tests，antimicrobial susceptibility testing，16S rRNAgene sequencing，phylogenetic tree construction and virulence gene detection.Results showed that one strain of Trueperell pyogenes（a Gram-positive， short-rod-shapedbacterium）was isolatedfromthelung tissue.Biochemical tests indicatedthatthe isolate was positive for lactose，maltose，and glucose fermentation，but negative for sucrose，rhamnose，and mannitol utilization.16S rRNA gene sequencing revealed more than 99 % homology between the Vulpes zerda isolate and reference strains of T. pyogenes from swine and cattle，clustering within the same branch onthe phylogenetic treeVirulence gene detection confirmed the presence of at least six virulence genes： Plo-g2 ， Plo-gl ， NanP ，FimA，FimE，and FimC Antimicrobial susceptibilitytestingdemonstrated sensitivitytomultipleantibiotics tested，withresistanceobserved onlytotwo sulfonamideantibiotics（sulfamethoxazole andtrimethoprim）.This study enriches the characterizationof Trueperella pyogenes derived from Vulpes zerda，providing a reference for research on wildlifepathogens.

Keywords：Vulpes zerda;Trueperella pyogenes；Drugsensitivity test;16S rRNA

耳廓狐（Vulpeszerda）是一种具有显著形态特征的小型狐类，分类学上属于食肉目犬科狐属，该物种主要分布于北非至亚洲西奈半岛的沙漠及半沙漠区域，表现出夜行性和杂食性。（剩余8635字）