不同冻存体系冷冻保存鸡原始生殖细胞(PGCs）的比较研究

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中图分类号：S814.8 文献标识码：A 文章编码：1005-8567（2025）06-0041-08

Comparative Study of Different Cryopreservation Systems for Chicken Primordial Germ Cells

SUNShengrui  ，ZHUYing，ZHAOHaoyi，HEYanhua，ZHAO Zhifeng， LUO Chenglong， SHU Dingming， ZOU Xian （1.IInstituteofAnimalScience，Guangdong AcademyofAgricultural Sciences & StateKeyLaboratoryofSuineand Poultry Breeding Industry& Key Laboratoryof Animal Nutrition andFeed Sciencein South China，Ministryof Agriculture and Rural Affairs，Guangzhou Guangdong 510640; （2.Huazhong Agricultural University，Wuhan Hubei 430000） Abstract：To identify the optimal cryopreservation protocol for chicken primordial germ cels （PGCs），eight cryopreservation solutions-including both literature-reportedand commerciallyavailable formulations-were evaluated.Two differentcellconcentrations werecryopreserved under identical culture conditionsand processing method.Post-thawanalyses，including trypan blue exclusion assay，CCK-8cell proliferation assay，andapoptosis detectio were used toperformed toassess cellviabilityand status in each group，thereby evaluating theperformance of the eight cryoprotectantsateach cellconcentration.The results showed thatt Cryoprotectant7yielded the highest number of viable cells at a concentration of 1.37 × 1 06 cells/mL，reaching 1.14×106 ，with 74.8% of cell being negative forbothfluorescein isothiocyanate（FITC）and propidium iodide（PI）.Incontrast，Cryoprotectant1yielded the highest number of viablecellsat 2.75×106cells/mL，reaching2.60×106，with 75.2% ouble-negative cells.However， Cryoprotectants II and M showed the least favorable outcomes.These findings indicate that the effectiveness of cryoprotectants varies with cell concentration.The most suitable solution for cryopreserving PGCs at 1.37 × 1 06 cells/mL in 250μL was Solution M （Saichang Biological Solution）.For cryopreservation solution PGCs at 2 .75×106 cells/mL， the optimal formulation was SolutionI （basal medium：chicken serum：DMSO CaCl=81.25%：10%：8%：0.75%）.

Keywords：Chicken;Primitive germcells;Frozen preservation;Poultrypreservation

现有的保种技术中，活体保种受到近亲繁殖、物种衰退和疾病威胁等问题的困扰，而冷冻保种技术可避免这些问题，实现跨时空的品种复苏。（剩余9990字）