广东小耳花猪精原干细胞体外培养体系的建立

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中图分类号：S814.8 文献标识码：A 文章编码：1005-8567（2025）06-0049-06

Establishment of in Vitro Culture System of Spermatogonial Stem Cells Isolated from Testis Samples of Guangdong Small-spotted Pigs

XIN Haiyun  ， ZHOU Shimeng，MENG Fanming

（1.State KeyLaboratoryofSuine andPoultry Breeding Industry，Guangdong KeyLaboratoryofAnimal Breedingand Nutrition，InstituteofAnimal Science，GuangdongAcademyofAgricultural Sciences，Guangzhou Guangdong510640;

2.College of Animal Science，South China Agricultural University， Guangzhou Guangdong 510642;

3.QingyuanLongfa PigBreeding Co.，Ltd，QingyuanGuangdong511500）

Abstract： The study aimed to study the characterization of spermatogonial stem cells （SSCs）from Guangdong Small-spottedPig testisinvitroafterseparation，purificationand invitroculture.Theinvestigationemployed enzyme digestion，density gradient centrifugation，anddiferential adhesionmethodsforcell enrichment，and cell immunofluorescence staining wasused to assess separation eficiency，cellproliferationand differentiation.The results indicatedthatthecombination of three separationmethodsefectively improved the purityof SSCs，which were successfullyproliferated in vitro，forming distinct cellcolonies byday14，thencellcolonies proliferation was significantly increasedbyday21.Immunostaining showed thatthe SSCs maker，UCHL-1was expressed highly within 28 days，while the expression of C-kit on day 56 indicated cell differentiation.

Keywords： Guangdong Small-spotted Pig； Testis； Spermatogonial stem cells; Immunofluorescence staining;UCHL-1

精原干细胞（spermatogonial stemcells，SSCs）是雄性哺乳动物精子发生的基础，为雄性动物生育能力提供保障。（剩余8205字）