分散固相萃取-高效液相色谱-串联质谱法测定茶叶中丙烯酰胺及风险评估研究

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中图分类号：TB9 文献标志码：A 文章编号：1674-5124（2025）09-0083-08

Abstract：An effective method was developed for the determination of acrylamide in tea by liquid chromatography-tandem mass spectrometry with dispersive solid-phase extraction combined. The sample was soaked in water and extracted with pure acetonitrile， and purified by 900mgMgSO4 300mg PSA， 300mg （204号 C18， 90mg GCB， 300mg SiLica. After separation on a XSELECT HSS T3 （2.5μm， 3.0mm×100 （20号 mm）column， the analytes were detected by LC-MS/MS in the multiple reaction monitoring（MRM） mode and quantified by internal standard method of solvent standard curve. Good linearity was obtained for acrylamide in the linear range of 0.5-250.0μg/L ，with correlation coefficient（ r2 ）larger than O.999，The limit of quantitation（LOQ） was 1.0μg/kg .The average recoveries at three levels of 10，20， 50μg/kg ranged from 90.4% to 101.3% ，respectively. The relative standard deviations（RSDs， n=6 ）ranged from 0.9% to 3.3% .280 samples were detected and 244 samples were found to contain acrylamide with a concentration ranging from ND to 305.7μg/kg .However， this method have certain advantageous such as simple operation， high sensitivity， and good accuracy.In conclusion，this method is suitable for the determination of acrylamide in tea.The assessment of the exposure risk of acrylamide in tea using the exposure limit method （MOE） indicates that there may be a high exposure risk under extreme ingestion conditions， which should be of concern to relevant departments.

Keywords： tea; dispersive solid-phase extraction; acrylamide; liquid chromatography-tandem mass spectrometry; risk assessment

0引言

丙烯酰胺（AA）是一种无色无味的小分子化合物，其在食品中产生的途径源自加热工艺过程中的美拉德反应。（剩余13905字）