刺槐蜂蜜的高特异性PCR检测技术体系的建立

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中图分类号：R126.4 文献标志码：A文章编号：1674-5124（2025）07-0104-07

Abstract：Honey is rich in nutrientsand widely cherished.As the world's largest honey producerand consumer， China faces a growing issue with adulterated honey entering the market due to increased demand. Existing nucleic acid detection systems for honey plants often fail to distinguish related species due to the lack of target specificity. This study employed bioinformatics methods to addressthis isue by comparing the highthroughput sequencing data of Robinia pseudoacacia with the genomes of related species and the NCBI NT nucleic acid database. We successfully identified a 3141 bp genome-specific DNA fragment of R. pseudoacacia. Based on this DNA fragment， we designed a PCR primer pair， RpsF1/RpsR13，with an amplification length of 257 bp. We then established a PCR system for the qualitative detection of locust honey. This system effectively distinguishes R. pseudoacacia from related Leguminosae species，such as Erythrina variegata. The system's sensitivity is notable， detecting a minimum template amount of 8.55 ag in a 20μL （2号 reaction， equivalent to 2.0 DNA copies. Testing of 20 locust honey samples yielded a 100% positive detection rate，demonstrating the system's high specificity and sensitivity.This new PCR detection assay ofers a robust technical solution for identifying authentic honey，thus helping to address the issue of adulterated honey in the market.

Keywords： honey; Robinia pseudoacacia; specific DNA fragment; PCR; detection assay

0 引言

蜂蜜是由蜜蜂采集植物的花蜜或蜜露与自身分泌物结合后酿造而成的天然甜味物质，是我国家庭常备食品，富含葡萄糖、果糖和蔗糖等多种营养素，及丰富的氨基酸、维生素、活性酶、类黄酮、多酚类、类胡萝卜素等活性物质及多种矿物质[1]。（剩余10762字）