烟草SRS基因家族鉴定与非生物胁迫诱导表达分析

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中图分类号：S572 文献标识码：A文章编号：1007-5119（2025）02-0083-10

，邓智超1,3，吴荣荣¹²，刘涛1,3，（1. 266101；2.青岛农业大学，青岛266109；3.中国农业科学院研究生院，北京 ）

Identification of Tobacco SRS Gene Family and Abiotic Stress-induced Expression Analysis

YANG Yalun12, DENG Zhichao1.3, DONG Ruonan1, PAN Xiaolu1,3, WU Rongrong1,2, LIU Tao1,³, GAO Xiaoming1* (1.InstituteofTobaccoResearchofCAAS,Qingdao266101,China;2.Qngdao Agricultural UniversityQingdao266109,Cina; 3. Graduate School of Chinese Academy of Agricultural Sciences, Beijing 10oo81, China)

Abstract: The SH-related sequence(SS)gene family encodes aclass of plant-specific zincfinger protein transcriptionfactors hat playcrucial roles inregulating plant growth,developmentandstressresponses.Toexplorethefunctionofte SRSgenefamily n tobacco,18NSRSgeneswereidentifiedfromthetobacogenomedatabaseanddesignatedasNSRS1toNtSRS18.Bioinformatics analyseswereconductedtoinvestigatetheirproteincharacteristics，subcelularlocalization,phylogeneticrelationships，gene structures,andooterentditoallyprioaesoilysdeenttisssdsel theirresponses todrought,salt,andlowtemperaturestresswereanalyzedusingqRT-CR.Resultsrevealedthat NtSRSproteins containedtheconservedDUF702domain,withmostlocalizedin the nucleus.Phylogeneticanalysis clusteredtheNtSRSfamilyinto 5 subfamilies，with membersofthesamesubfamilyexhibitinghighlyconservedgeneand protein structures.Promoteranalysis identifiednumeroushormone-responsiveelementsand stres-responsive elementswithintheregulatoryregionsof NtSRSgenes. Expresion proilingshowed thatNtSRSgnes exhbitedsgnificant tssuespeciic expresson,withosthighlyexpressd inflower budsand stems.NotablyNSRS3and NSRS16 were stronglyinducedunderdrought,salt,andlowtemperature strescoditios. OverallthisstudyprovidesacomprehensivecharacterzationofthetobaccoSRSgenefamilyofferingatheoreticalbasisforthe genetic improvement of tobacco germplasm resources.

Keywords: tobacco; SRS gene family; abiotic stress; expression pattern analysis

SRS基因家族又称为短节间相关序列[shortinternodes(SHI）-related sequences]基因家族或者STY(STYLISH)基因家族，该家族基因编码一种植物特有的锌指蛋白转录因子I。（剩余14945字）